Fang Xinmei, Qin Kai, Li Shujiang, Han Shan, Zhu Tianhui, Fang Xinmei, Qin Kai
College of Forestry, Sichuan Agricultural University, Chengdu 611130, Sichuan Province, China.
College of Forestry, Sichuan Agricultural University, Chengdu 611130, Sichuan Province, China.
Genomics. 2020 Sep;112(5):3751-3761. doi: 10.1016/j.ygeno.2020.04.018. Epub 2020 May 3.
Many fungi in the Diaporthe genus across the world are pathogenic. Diaporthe capsici. is a pathogenic fungus that can infect peppers and walnuts, causing their death. The aim of this study was to develop a genomic resource to provide substantial data and a theoretical basis for research on molecular pathogenesis, transcriptome, proteome, and metabonome of D. capsici. The whole genome of D. capsici was sequenced using the PacBio RSII sequencing platform, and functional annotation was performed using different public databases. The genome was found to be 57.56 Mb in size, with an N50 contig size of 5,171,887 bp, and encodes 14,425 putative genes. This is the first genome-scale assembly and annotation for D. capsici, which is the eighth species in Diaporthe to be sequenced.
世界上许多间座壳属真菌具有致病性。辣椒间座壳是一种致病真菌,可感染辣椒和核桃,导致其死亡。本研究的目的是开发一种基因组资源,为辣椒间座壳的分子发病机制、转录组、蛋白质组和代谢组研究提供大量数据和理论基础。使用PacBio RSII测序平台对辣椒间座壳的全基因组进行测序,并使用不同的公共数据库进行功能注释。发现该基因组大小为57.56 Mb,N50重叠群大小为5,171,887 bp,编码14,425个推定基因。这是辣椒间座壳的首次全基因组组装和注释,它是间座壳属中第八个进行测序的物种。