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基因组和转录组分析为了解SR21的ω-3长链多不饱和脂肪酸生物合成提供了线索。

Genome and Transcriptome Analyses Provide Insight Into the Omega-3 Long-Chain Polyunsaturated Fatty Acids Biosynthesis of SR21.

作者信息

Liang Limin, Zheng Xuehai, Fan Wenfang, Chen Duo, Huang Zhen, Peng Jiangtao, Zhu Jinmao, Tang Weiqi, Chen Youqiang, Xue Ting

机构信息

The Public Service Platform for Industrialization Development Technology of Marine Biological Medicine and Products of the State Oceanic Administration, Center of Engineering Technology Research for Microalga Germplasm Improvement of Fujian, Fujian Key Laboratory of Special Marine Bioresource Sustainable Utilization, Key Laboratory of Developmental and Neural Biology, Southern Institute of Oceanography, College of Life Sciences, Fujian Normal University, Fuzhou, China.

Institute of Oceanography, Marine Biotechnology Center, Minjiang University, Fuzhou, China.

出版信息

Front Microbiol. 2020 Apr 16;11:687. doi: 10.3389/fmicb.2020.00687. eCollection 2020.

DOI:10.3389/fmicb.2020.00687
PMID:32373097
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7179369/
Abstract

sp. is the best natural resource for omega-3 long-chain polyunsaturated fatty acids. We report a high-quality genome sequence of SR21, which has a 63 Mb genome size, with a contig N50 of 2.67 Mb and 6,838 protein-coding genes. Phylogenomic and comparative genomic analyses revealed that DHA-producing and strains were highly similar and possessed similar genes. Analysis of the fatty acid synthase (FAS) for LC-PUFAs production results in the annotation of all genes in map00062 and map01212. A gene cluster and 10 ORFs related to PKS pathway were found in the genome. 1,402 differentially expressed genes (DEGs) of the treated groups (0.5 g/L yeast extract) were identified by comparing with the control groups (1.0 g/L yeast extract) at 36 h. A weighted gene coexpression network analysis revealed that 2 of 7 modules correlated highly with the fatty acid and DHA contents. The DEGs and transcription factors were significantly correlated with fatty acid biosynthesis, including MYB, Zinc Finger and ACOX. The results showed that these hub genes are regulated by genes involved in fatty acid biosynthesis pathways. The results providing an important reference for further research on promoting fatty acid and DHA accumulation in SR21.

摘要

某物种是ω-3长链多不饱和脂肪酸的最佳天然资源。我们报告了SR21的高质量基因组序列，其基因组大小为63 Mb，重叠群N50为2.67 Mb，有6838个蛋白质编码基因。系统发育基因组学和比较基因组学分析表明，产DHA的菌株高度相似且拥有相似基因。对用于生产LC-PUFA的脂肪酸合酶（FAS）的分析导致在map00062和map01212中对所有基因进行注释。在基因组中发现了一个基因簇和10个与聚酮合酶途径相关的开放阅读框。通过在36小时时将处理组（0.5 g/L酵母提取物）与对照组（1.0 g/L酵母提取物）进行比较，鉴定出1402个差异表达基因（DEG）。加权基因共表达网络分析表明，7个模块中的2个与脂肪酸和DHA含量高度相关。差异表达基因和转录因子与脂肪酸生物合成显著相关，包括MYB、锌指蛋白和ACOX。结果表明，这些枢纽基因受脂肪酸生物合成途径中涉及的基因调控。这些结果为进一步研究促进SR21中脂肪酸和DHA积累提供了重要参考。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53a2/7179369/b192a7e88ae6/fmicb-11-00687-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53a2/7179369/87610394cbc2/fmicb-11-00687-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53a2/7179369/67a232e6e5d2/fmicb-11-00687-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53a2/7179369/7793bfb569dc/fmicb-11-00687-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53a2/7179369/b192a7e88ae6/fmicb-11-00687-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53a2/7179369/87610394cbc2/fmicb-11-00687-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53a2/7179369/8325d4442530/fmicb-11-00687-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53a2/7179369/9fea5b3afc23/fmicb-11-00687-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53a2/7179369/ce048d1c42db/fmicb-11-00687-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53a2/7179369/f40770ca816c/fmicb-11-00687-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53a2/7179369/67a232e6e5d2/fmicb-11-00687-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53a2/7179369/7793bfb569dc/fmicb-11-00687-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53a2/7179369/b192a7e88ae6/fmicb-11-00687-g008.jpg

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