Buranrat Benjaporn, Noiwetch Sirintra, Suksar Tippaporn, Ta-Ut Aphimook
MD Program, Faculty of Medicine, Mahasarakham University, Muang District, Maha Sarakham, 44000, Thailand.
Applied Thai Traditional Medicine Program, Faculty of Medicine, Mahasarakham University, Muang District, Maha Sarakham, 44000, Thailand.
J Pharm Anal. 2020 Apr;10(2):187-193. doi: 10.1016/j.jpha.2020.02.003. Epub 2020 Mar 5.
In this study, we investigated how leaf and fruit extracts affect the viability and migration of MCF-7 breast cancer cells and the mechanisms of action responsible for these effects. MCF-7 cells treated with the extracts were examined using the sulforhodamine B, colony formation and caspase 3 activity assays, and by Western blotting. extracts were found to inhibit MCF-7 cell growth in a concentration- and time-dependent manner, with 48 h IC values of 57.02 ± 2.85 μg/mL and 131.3 ± 19.2 μg/mL for leaf and fruit extracts, respectively. Further, the leaf extract caused a reduction in MCF-7 cell viability, induction of MCF-7 cell apoptosis and ROS formation, and an increase in caspase 3 activity. Also, the two extracts inhibited MCF-7 cell migration and reduced both and gene expression and MMP9 protein expression. Additionally, extracts greatly reduced expression of the cell cycle regulatory protein Rac1 in the mevalonate pathway. In summary, leaf and fruit extracts reduce breast cancer cell growth, cell viability and cell migration. constituents could, therefore, be useful for augmenting the activity of chemotherapeutic drugs employed to treat breast cancer.
在本研究中，我们调查了叶提取物和果实提取物如何影响MCF-7乳腺癌细胞的活力和迁移以及导致这些作用的作用机制。使用磺酰罗丹明B、集落形成和半胱天冬酶3活性测定以及蛋白质免疫印迹法对用提取物处理的MCF-7细胞进行检测。发现提取物以浓度和时间依赖性方式抑制MCF-7细胞生长，叶提取物和果实提取物的48小时半数抑制浓度（IC）值分别为57.02±2.85μg/mL和131.3±19.2μg/mL。此外，叶提取物导致MCF-7细胞活力降低、MCF-7细胞凋亡和活性氧形成增加以及半胱天冬酶3活性增加。而且，两种提取物均抑制MCF-7细胞迁移，并降低 和 基因表达以及基质金属蛋白酶9（MMP9）蛋白表达。此外，提取物极大地降低了甲羟戊酸途径中细胞周期调节蛋白Rac1的表达。总之，叶提取物和果实提取物可降低乳腺癌细胞的生长、细胞活力和细胞迁移。因此，其成分可能有助于增强用于治疗乳腺癌的化疗药物的活性。
