Geng Vivanco Rocio, Cardoso Renata Silva, Sousa Ana Beatriz Silva, Chinelatti Michelle Alexandra, Vincenti Sergio Augusto de Freitas, Tonani-Torrieri Rafaella, Pires-de-Souza Fernanda de Carvalho Panzeri
Department of Dental Materials and Prosthodontics, Ribeirão Preto School of Dentistry, University of São Paulo, Av do Café s/n, 14040-904, Ribeirão Preto, SP, Brazil.
School of Dentistry, University of Ribeirão Preto, Av Costábile Romano, 2.201, 14096-900, Ribeirão Preto, SP, Brazil.
Heliyon. 2020 Apr 28;6(4):e03871. doi: 10.1016/j.heliyon.2020.e03871. eCollection 2020 Apr.
This study evaluated the influence of thermo-mechanical cycling (TMC) on the bond strength (BS) of a universal adhesive system (UAS - Adper Single Bond Universal, 3M ESPE) to dentin treated or not with 0.2% chlorhexidine (CHX).
Eighty human molars were flattened until reach the dentin and separated into 4 groups according to the bonding protocol: ENR Group: 37% phosphoric acid + 3-step etch-and-rinse adhesive system (ENR); UAS Group: UAS in self-etch mode; ENR + CHX Group: 37% phosphoric acid + CHX + ENR; UAS + CHX Group: CHX + UAS in self-etch mode. After treatments, teeth were restored (Filtek Z350, 3M ESPE). Samples (n = 10) were submitted to aging process: stored in distilled water at 37°C/30 days or TMC (ERIOS - 98N/1.6Hz + thermal cycling 5/37/55 °C - 1,200,000 cycles). Specimens were sectioned into sticks (1.0 mm) and submitted to the microtensile test (Mechanical Test Machine - 0.5 mm/min). Fracture patterns and hybrid layer integrity were analyzed under Stereomicroscope and Scanning Electron Microscopy (SEM).
The BS results (3-way ANOVA, Bonferroni's test, α = 5%) showed that groups treated with CHX presented higher BS values than control groups; significant in all cases (p < .05), except for ENR submitted to TMC (p > .05). When CHX was applied and samples were cycled, UAS revealed higher BS (p < .05) than ENR. After TMC, cohesive fractures increased for UAS, regardless of CHX application. SEM analysis demonstrated different hybridization patterns for the adhesive systems tested.
The performance of the universal adhesive system used in self-etch mode was better than that of the 3-step etch-and-rinse adhesive system.
Universal adhesive systems have been developed in order to simplify the dentin hybridization protocol. It is important to determine the longevity of the adhesive interface using these bonding materials after chewing.
本研究评估了热机械循环（TMC）对一种通用粘结系统（UAS - Adper Single Bond Universal，3M ESPE）与经0.2%氯己定（CHX）处理或未处理的牙本质之间粘结强度（BS）的影响。
将80颗人类磨牙磨平直至露出牙本质，并根据粘结方案分为4组：ENR组：37%磷酸 + 三步酸蚀冲洗粘结系统（ENR）；UAS组：自酸蚀模式下的UAS；ENR + CHX组：37%磷酸 + CHX + ENR；UAS + CHX组：CHX + 自酸蚀模式下的UAS。处理后，用牙齿修复材料（Filtek Z350，3M ESPE）进行修复。样本（n = 10）进行老化处理：在37°C蒸馏水中储存30天或进行TMC（ERIOS - 98N/1.6Hz + 热循环5/37/55°C - 1,200,000次循环）。将样本切成小棒（1.0 mm）并进行微拉伸试验（机械试验机 - 0.5 mm/min）。在体视显微镜和扫描电子显微镜（SEM）下分析断裂模式和混合层完整性。
粘结强度结果（三因素方差分析，Bonferroni检验，α = 5%）表明，用CHX处理的组的粘结强度值高于对照组；在所有情况下均有显著差异（p <.05），但ENR组进行TMC处理的除外（p >.05）。当应用CHX且样本进行循环处理时，UAS的粘结强度高于ENR（p <.05）。TMC处理后，无论是否应用CHX，UAS的内聚性断裂均增加。SEM分析显示所测试的粘结系统具有不同的混合模式。
自酸蚀模式下使用的通用粘结系统的性能优于三步酸蚀冲洗粘结系统。
开发通用粘结系统是为了简化牙本质混合方案。确定咀嚼后使用这些粘结材料时粘结界面的耐久性很重要。
