Dong Pengfei, Tu Xiaoyu, Liang Zizheng, Kang Byung-Ho, Zhong Silin
The South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China.
State Key Laboratory of Agrobiotechnology, School of Life Sciences, The Chinese University of Hong Kong, Hong Kong, China.
J Exp Bot. 2020 Aug 17;71(17):5119-5128. doi: 10.1093/jxb/eraa220.
Chromatin is the main carrier of genetic information and is non-randomly distributed within the nucleus. Next-generation sequence-based chromatin conformation capture technologies have enabled us to directly examine its three-dimensional organization at an unprecedented scale and resolution. In the best-studied mammalian models, chromatin folding can be broken down into three hierarchical levels, compartment, domains, and loops, which play important roles in transcriptional regulation. Although similar structures have now been identified in plants, they might not possess exactly the same functions as the mammalian ones. Here, we review recent Hi-C studies in plants, compare plant chromatin structures with their mammalian counterparts, and discuss the differences between plants with different genome sizes.
染色质是遗传信息的主要载体，在细胞核内呈非随机分布。基于新一代测序的染色质构象捕获技术使我们能够以前所未有的规模和分辨率直接研究其三维结构。在研究得最为透彻的哺乳动物模型中，染色质折叠可分为三个层次水平，即区室、结构域和环，它们在转录调控中发挥着重要作用。尽管目前在植物中已鉴定出类似结构，但它们可能并不具备与哺乳动物结构完全相同的功能。在此，我们综述了近期植物中的Hi-C研究，将植物染色质结构与其哺乳动物对应物进行比较，并讨论了不同基因组大小植物之间的差异。
