sp. nov. a novel actinobacterium isolated from the stony coral .

A novel Gram-stain-positive, aerobic, non-motile actinobacterium, designated strain E2A, was isolated from a coral sample and examined using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain E2A formed a distinct phyletic lineage in the genus and was closely related to CCTCC AA 2012022 (96.4 %) and CCTCC AA 2010012 (95.3 %). The isolate grew at 15-35 °C, pH 5-12 and in the presence of 1-16 % (w/v) NaCl. The cell-wall diamino acid was -DAP. Major fatty acids identified were -C, -C and C ω8c. The predominant menaquinone was MK-9(H). The polar lipids detected were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylmethylethanolamine, one unidentified glycolipid, one unidentified phospholipid and one unidentified aminolipid. The genomic DNA G+C content was 68.6 mol%. Based on the data from the polyphasic taxonomic study reported here, strain E2A represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is E2A=(JCM 31844=MCCC 1A17150).