Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Japan Collection of Microorganisms, RIKEN BioResource Center, Ibaraki 305-0074, Japan.
Int J Syst Evol Microbiol. 2020 May;70(5):3316-3322. doi: 10.1099/ijsem.0.004170.
An endophytic actinomycete, strain 3MP-10, isolated from the root of was taxonomically studied based upon polyphasic approaches. This strain formed spiral spore chains on aerial mycelia. ll-Diaminopimelic acid, glucose and ribose were found in the whole-cell hydrolysates. It belonged to the genus and was closely related to DSM 42101 (98.9 %) and JCM 16909 (98.6 %) based on 16S rRNA gene sequence analysis results. The major menaquinones were MK-10(H), MK-10(H) and MK-9(H). The predominant cellular fatty acids were isoC, anteiso-C and anteisoC. The detected phospholipids were diphosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol, phosphatidylethanolamine and phosphatidylglycerol. Strain 3MP-10 had a genome size of 7.2 Mb with a genome G+C content of 73.4 mol%. Results of genome-based similarity analysis revealed ANIb values of 84.94 and 84.77 %, ANIm values of 88.01 and 87.92 %, and dDDH values of 29.9 and 29.6 % when compared with DSM 42101 and JCM 16909, respectively. Based on the polyphasic approach, digital DNA-DNA relatedness and average nucleotide identity, we propose that the novel actinomycete represents a novel species, , with type strain 3MP-10 (=JCM 33328=TISTR 2646).
一株内生放线菌，菌株 3MP-10，从植物根部分离得到，基于多相分类方法进行了分类学研究。该菌株在气生菌丝上形成螺旋孢子链。全细胞水解物中含有 ll-二氨基庚二酸、葡萄糖和核糖。它属于 属，与 DSM 42101（98.9%）和 JCM 16909（98.6%）最为相近，基于 16S rRNA 基因序列分析结果。主要的menaquinones 为 MK-10(H)、MK-10(H)和 MK-9(H)。主要的细胞脂肪酸为 isoC、anteiso-C 和 anteiso-C。检测到的磷脂有双磷脂酰甘油、磷脂酰肌醇甘露糖苷、磷脂酰肌醇、磷脂酰乙醇胺和磷脂酰甘油。菌株 3MP-10 的基因组大小为 7.2 Mb，基因组 G+C 含量为 73.4 mol%。基于基因组相似性分析的结果表明，与 DSM 42101 和 JCM 16909 相比，ANIb 值分别为 84.94%和 84.77%，ANI m 值分别为 88.01%和 87.92%，dDDH 值分别为 29.9%和 29.6%。基于多相分类方法、数字 DNA-DNA 相关值和平均核苷酸同一性，我们建议该新型放线菌代表一个新种， ，其模式菌株为 3MP-10（=JCM 33328=TISTR 2646）。
