State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Microbial Culture Collection Center (GDMCC), Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China.
Int J Syst Evol Microbiol. 2020 May;70(5):3553-3558. doi: 10.1099/ijsem.0.004216.
A polyphasic taxonomic study was undertaken to clarify the exact position of the type strain cai42 of the species Pan . 2015. The results of the 16S rRNA gene sequence analysis indicated that the two sequences from cai42 shared 99.6 and 99.7 % similarity to that of the type strain SP32 of the species and formed a coherent clade in the phylogenetic tree. Whole genomic comparison between cai42 and SP32 yielded a digital DNA-DNA hybridization estimate of 36.3 %, an average nucleotide identity of 88.8 % and an average amino acid identity of 89.8 %, clearly indicating that the two strains should belong to two genospecies of the same genus. The close relationship between the two strains was underpinned by the results of genome-based phylogenetic analysis. Although cai42 and SP32 shared similar physiological and biochemical properties, some striking differences, such as mobility, the temperature range for growth and the polar lipid components, could distinguish them as separate species. Therefore, the comparative phenotypic and genotypic analyses supported the incorporation of into the genus as nom. nov. with the type strain cai42 (=CGMCC 1.12518=LMG 27406).
进行了多相分类学研究，以明确物种 Pan. 2015 的模式菌株 cai42 的确切位置。16S rRNA 基因序列分析结果表明，cai42 的两个序列与物种 SP32 的模式菌株的序列具有 99.6%和 99.7%的相似性，并在系统发育树中形成了一个连贯的分支。cai42 和 SP32 之间的全基因组比较得出数字 DNA-DNA 杂交估计值为 36.3%，平均核苷酸同一性为 88.8%，平均氨基酸同一性为 89.8%，这清楚地表明这两个菌株应属于同一属的两个不同种。两个菌株之间的密切关系得到了基于基因组的系统发育分析结果的支持。尽管 cai42 和 SP32 具有相似的生理生化特性，但一些显著的差异，如运动性、生长温度范围和极性脂成分，可以将它们区分开来，作为不同的种。因此，比较表型和基因型分析支持将 归入 属，作为新种，其模式菌株为 cai42(=CGMCC 1.12518=LMG 27406)。
