Frontier Institute of Science and Technology, and State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an, 710049, China.
Department of Orthopaedics, The First Affiliated Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an, 710061 China.
Biomacromolecules. 2020 May 11;21(5):1841-1852. doi: 10.1021/acs.biomac.9b01732.
As the abuse of antibiotics continues to increase, the emergence of antibiotic resistance and unknown drug-resistant bacterial infections pose a great threat on people worldwide. In this work, we aimed to develop a series of injectable antimicrobial conductive hydrogels based on glycidyl methacrylate functionalized quaternized chitosan (QCSG), gelatin methacrylate (GM), and graphene oxide (GO) for drug-resistant bacterial disinfection and infectious wound healing. The rheology, morphology, mechanical properties, and electrical and photothermal properties of the hydrogels were characterized. Furthermore, the good in vitro and in vivo intrinsic antibacterial, photothermal antibacterial, and antibiotics released antibacterial properties of this multiantibacterial hydrogel were verified. The good biocompatibility of these hydrogels was also investigated by cytocompatibility, hemocompatibility, and histocompatibility tests. In the drug-resistant Methicillin-resistant (MRSA) infected mouse full-thickness defect model, the wound closure rate, the length of dermal tissue gap, number of blood vessels and hair follicles in hematoxylin-eosin (HE) staining, the amount of collagen in Masson staining, and the related cytokines for the expression of inflammation (interleukin-6, IL-6) and regeneration of blood vessels (vascular endothelial growth factor, VEGF) in immunofluorescence were all further studied. All the results demonstrated the better wound healing effect of these multiantibacterial injectable conductive hydrogel in infectious skin tissue defect repair, indicating their great potential for infected wound healing.
随着抗生素的滥用不断增加，抗生素耐药性和未知的耐药细菌感染的出现对全球人类构成了巨大威胁。在这项工作中，我们旨在开发一系列基于甲基丙烯酰氧基丙基三甲基氯化铵壳聚糖（QCSG）、甲基丙烯酸明胶（GM）和氧化石墨烯（GO）的可注射抗菌导电水凝胶，用于耐药细菌的消毒和感染性伤口愈合。对水凝胶的流变性、形态、机械性能、电学和光热性能进行了表征。此外，还验证了这种多抗菌水凝胶在体外和体内的固有抗菌、光热抗菌和抗生素释放抗菌性能。通过细胞相容性、血液相容性和组织相容性试验研究了这些水凝胶的良好生物相容性。在耐甲氧西林金黄色葡萄球菌（MRSA）感染的小鼠全层缺陷模型中，进一步研究了伤口闭合率、皮肤组织间隙长度、苏木精-伊红（HE）染色的血管和毛囊数量、Masson 染色的胶原蛋白量以及免疫荧光中用于炎症表达的相关细胞因子（白细胞介素 6，IL-6）和血管再生（血管内皮生长因子，VEGF）。所有结果均表明，这些多抗菌可注射导电水凝胶在感染性皮肤组织缺陷修复中具有更好的伤口愈合效果，表明它们在感染性伤口愈合方面具有巨大的潜力。
