School of Dentistry, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
College of Medicine, Orthopaedic Research Center, Kaohsiung Medical University, Kaohsiung, Taiwan.
Oral Dis. 2020 Oct;26(7):1474-1482. doi: 10.1111/odi.13409. Epub 2020 Jun 3.
Oral submucous fibrosis (OSMF) is a chronic inflammatory disease and a potentially malignant oral disorder. However, the best therapeutic treatment for OSMF remains uncertain. Our previous study showed that photobiomodulation (PBM) therapy and forskolin could reduce arecoline-induced fibrosis reactions via the cAMP pathway. The present study aimed to establish an animal model of areca nut extract (ANE)-induced OSMF and to evaluate the therapeutic potential of PBM and forskolin for ANE-induced OSMF.
The mice were divided into five groups. The buccal tissues were harvested for histomorphological analysis and immunoblotting.
Our results showed that PBM significantly reduced the development of ANE-induced OSMF, quantified by changes in submucosal layer thickness and collagen deposition. Additionally, PBM could extensively reduce the protein expression of the fibrotic marker genes alpha-smooth muscle actin (α-SMA) and connective tissue growth factor (CTGF) in buccal submucous lesions. However, forskolin treatment significantly decreased the protein expression of fibrotic marker genes but slightly decreased the observed histomorphological changes.
We established an ANE-induced OSMF mouse model, which also provided a model for the development of a therapeutic treatment for OSMF. The anti-fibrotic effects of PBM and forskolin may be useful for clinical interventions.
口腔黏膜下纤维性变（OSMF）是一种慢性炎症性疾病，也是一种潜在的恶性口腔疾病。然而，OSMF 的最佳治疗方法仍不确定。我们之前的研究表明，光生物调节（PBM）疗法和 forskolin 可以通过 cAMP 通路减少槟榔碱诱导的纤维化反应。本研究旨在建立槟榔提取物（ANE）诱导的 OSMF 动物模型，并评估 PBM 和 forskolin 对 ANE 诱导的 OSMF 的治疗潜力。
将小鼠分为五组。采集颊组织进行组织形态学分析和免疫印迹。
我们的结果表明，PBM 显著减轻了 ANE 诱导的 OSMF 的发展，这可以通过粘膜下层厚度和胶原沉积的变化来量化。此外，PBM 可以广泛减少颊粘膜下病变中纤维化标记基因α-平滑肌肌动蛋白（α-SMA）和结缔组织生长因子（CTGF）的蛋白表达。然而，forskolin 治疗显著降低了纤维化标记基因的蛋白表达，但对观察到的组织形态学变化影响较小。
我们建立了 ANE 诱导的 OSMF 小鼠模型，该模型也为 OSMF 的治疗方法的发展提供了模型。PBM 和 forskolin 的抗纤维化作用可能对临床干预有用。
