Hattori S, Furuse A, Matsuda I
Department of Pediatrics, Kumamoto University Medical School, Japan.
Am J Nephrol. 1988;8(5):384-7. doi: 10.1159/000167622.
The specificity of IgG on the glomerular capillary wall was investigated in 3 patients with hepatitis B virus associated membranous glomerulonephritis. The immune deposits on the capillary walls were stained by immunofluorescent antibody against HBe antigen and IgG. The eluted fluid (0.02 M citrate buffer, pH 3.2) from renal biopsy slices contained significant activity of HBe antibody, but not of HBs and HBc antibodies. After elution, the disappearance of IgG on the capillary walls was confirmed by immunofluorescence. Heterologous complement activation with fresh guinea pig complement was positive in the glomerular capillary walls from all 3 patients. Our observations support the notion that this disease is caused by HBe antigen-anti-HBe immune complexes.
对3例乙型肝炎病毒相关性膜性肾小球肾炎患者肾小球毛细血管壁上IgG的特异性进行了研究。用抗HBe抗原和IgG的免疫荧光抗体对毛细血管壁上的免疫沉积物进行染色。肾活检切片洗脱液（0.02M柠檬酸盐缓冲液，pH 3.2）含有显著活性的HBe抗体，但不含有HBs和HBc抗体。洗脱后，通过免疫荧光证实毛细血管壁上IgG消失。用新鲜豚鼠补体进行的异种补体激活在所有3例患者的肾小球毛细血管壁上均呈阳性。我们的观察结果支持这种疾病是由HBe抗原-抗HBe免疫复合物引起的这一观点。
