Mahmoodzadeh Hosseini Hamideh, Hamzeh Pour Siavash, Amani Jafar, Jabbarzadeh Sima, Hosseinabadi Mostafa, Mirhosseini Seyed Ali
1Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
2Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
J Environ Health Sci Eng. 2020 Mar 26;18(1):297-302. doi: 10.1007/s40201-020-00467-y. eCollection 2020 Jun.
Aflatoxins are one of the most important mycotoxins, which have been classified as Group I carcinogenic compounds by the International Agency for Research on Cancer. This investigation aimed to examine the effect of Propolis on inhibition of the growth, aflatoxin production and expression of aflatoxin biosynthesis pathway genes.
A standard strain of (ATCC 15517) was used to perform antifungal susceptibility test, using a microdilution method in accordance with the CLSI M38-A2 guidelines. The aflatoxin concentrations in the control and treated media were determined by HPLC. Also, the quantitative changes in the level of , and genes expression in aflatoxin biosynthetic pathway were analyzed using Real-Time PCR method.
The results showed that the minimum inhibitory concentrations (MIC) of propolis was 100 µg/ml. The results showed that total levels of aflatoxin decreased from 386.1 ppm to 3.01 ppm at 50 µg/ml of propolis. In addition, quantitative real-time PCR analysis showed that the level of and genes expression was significantly decreased after treatment with propolis extract.
The findings reveal that propolis extract, have a significant inhibitory effect on important genes for aflatoxin biosynthesis pathway in aflatoxin production.
黄曲霉毒素是最重要的霉菌毒素之一，已被国际癌症研究机构列为I类致癌化合物。本研究旨在探讨蜂胶对黄曲霉生长、黄曲霉毒素产生以及黄曲霉毒素生物合成途径基因表达的抑制作用。
使用黄曲霉标准菌株（ATCC 15517），按照CLSI M38 - A2指南采用微量稀释法进行抗真菌药敏试验。通过高效液相色谱法测定对照培养基和处理后培养基中的黄曲霉毒素浓度。此外，采用实时荧光定量PCR法分析黄曲霉毒素生物合成途径中、和基因表达水平的定量变化。
结果显示蜂胶的最低抑菌浓度（MIC）为100μg/ml。结果表明，在50μg/ml蜂胶作用下，黄曲霉毒素总水平从386.1ppm降至3.01ppm。此外，实时荧光定量PCR分析表明，用蜂胶提取物处理后，和基因的表达水平显著降低。
研究结果表明，蜂胶提取物对黄曲霉毒素产生过程中黄曲霉毒素生物合成途径的重要基因具有显著抑制作用。
