Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, PR China.
Shanghai Health Commission Key Lab of Artificial Intelligence (AI)-Based Management of Inflammation and Chronic Diseases, Sino-French Cooperative Central Lab, Shanghai Pudong Gongli Hospital, Secondary Military Medical University, Shanghai 200135, PR China.
Clin Chim Acta. 2020 Sep;508:110-114. doi: 10.1016/j.cca.2020.05.019. Epub 2020 May 13.
We observe changes of the main lymphocyte subsets (CD16CD56、CD19、CD3、CD4、and CD8) in COVID-19-infected patients and explore whether the changes are associated with disease severity.
One-hundred and fifty-four cases of COVID-19-infected patients were selected and divided into 3 groups (moderate group, severe group and critical group). The flow cytometry assay was performed to examine the numbers of lymphocyte subsets.
CD3, CD4 and CD8 + T lymphocyte subsets were decreased in COVID-19-infected patients. Compared with the moderate group and the sever group, CD3, CD4 and CD8 T cells in the critical group decreased greatly (P < 0.001, P = 0.005 or P = 0.001).
Reduced CD3, CD4, CD8 T lymphocyte counts may reflect the severity of the COVID-19. Monitoring T cell changes has important implications for the diagnosis and treatment of severe patients who may become critically ill.
我们观察了 COVID-19 感染患者主要淋巴细胞亚群（CD16CD56、CD19、CD3、CD4 和 CD8）的变化，并探讨这些变化是否与疾病严重程度有关。
选择了 154 例 COVID-19 感染患者，并将其分为 3 组（中度组、重度组和危重组）。通过流式细胞术检测淋巴细胞亚群的数量。
COVID-19 感染患者的 CD3、CD4 和 CD8+T 淋巴细胞亚群减少。与中度组和重度组相比，危重组的 CD3、CD4 和 CD8 T 细胞显著减少（P<0.001，P=0.005 或 P=0.001）。
CD3、CD4、CD8 T 淋巴细胞计数减少可能反映了 COVID-19 的严重程度。监测 T 细胞的变化对诊断和治疗可能发展为危重症的重症患者具有重要意义。
