颞骨岩部的问题？对用于古代DNA分析的潜在偏差的思考。
The problem with petrous? A consideration of the potential biases in the utilization of for ancient DNA analysis.
作者信息
Charlton Sophy, Booth Thomas, Barnes Ian
机构信息
Department of Earth Sciences, Natural History Museum, London, UK.
PalaeoBARN, School of Archaeology, University of Oxford, Oxford, UK.
出版信息
World Archaeol. 2020 Jan 10;51(4):574-585. doi: 10.1080/00438243.2019.1694062. eCollection 2019.
Advances in NGS sequencing technologies, improved laboratory protocols and new bioinformatic workflows have seen huge increases in ancient DNA (aDNA) research on archaeological materials. A large proportion of aDNA work now utilizes the petrous portion of the temporal bone (, which is recognized as an excellent skeletal element for long-term ancient endogenous (host) DNA survival. This has been significant due to the often low endogenous content of other skeletal elements, meaning that large amounts of sequencing are frequently required to obtain sufficient genetic coverage. However, exclusive sampling of the petrous for aDNA analysis introduces a new set of potential biases into our scientific studies - and these issues are yet to be considered by ancient DNA researchers. This paper aims to outline the possible biases of utilizing petrous bones to undertake aDNA analyses and highlight how these complications may potentially be overcome in future research.
新一代测序（NGS）技术的进步、改进的实验室方案和新的生物信息学工作流程，使得对考古材料的古DNA（aDNA）研究有了大幅增长。现在，很大一部分aDNA工作都利用了颞骨的岩部（，它被认为是长期保存古代内源性（宿主）DNA的极佳骨骼元素。这一点很重要，因为其他骨骼元素的内源性含量往往较低，这意味着经常需要进行大量测序才能获得足够的基因覆盖度。然而，仅对岩部进行aDNA分析采样给我们的科学研究带来了一系列新的潜在偏差——而这些问题尚未被古DNA研究人员所考虑。本文旨在概述利用岩骨进行aDNA分析可能存在的偏差，并强调在未来研究中如何有可能克服这些复杂问题。
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