Dipartimento di Scienze Farmaceutiche, Università degli Studi di Perugia, Via del Liceo 1, 06123 Perugia, Italy.
Dipartimento di Scienze Farmaceutiche, Università degli Studi di Perugia, Via del Giochetto, 06122 Perugia, Italy.
Mater Sci Eng C Mater Biol Appl. 2020 Jul;112:110863. doi: 10.1016/j.msec.2020.110863. Epub 2020 Mar 19.
Alginate films containing pyrogenic silica supported silver nanoparticles were prepared as potential wound dressings. First silica supported silver nanoparticles (CAB-O-SIL-Ag) were prepared via solid state sintering route without the use of any solvent and reducing agent. The obtained composite was characterized by X-ray powder diffraction, transmission electron microscopy and UV-vis spectroscopy which evidenced the presence of 8-20 nm spherical silver nanoparticles uniformly distributed and grown on the silica surface. Then the CAB-O-SIL-Ag was used as filler to prepare alginate films by casting method and successive gelation. Films with two different silver concentrations were prepared. They showed good hydration properties and a very slow silver release. Films exhibited antimicrobial and antibiofilm activities against Staphylococcus aureus and Pseudomonas aeruginosa and showed no cytotoxicity towards human skin keratinocytes and human fibroblasts HuDe.
含热解法二氧化硅负载银纳米粒子的海藻酸盐膜被制备为潜在的伤口敷料。首先，通过无溶剂和还原剂的固态烧结法制备了负载银纳米粒子的热解法二氧化硅（CAB-O-SIL-Ag）。通过 X 射线粉末衍射、透射电子显微镜和紫外-可见光谱对所得复合材料进行了表征，结果表明，8-20nm 的球形银纳米粒子均匀分布并生长在二氧化硅表面。然后，将 CAB-O-SIL-Ag 用作填充剂，通过浇铸法和连续凝胶化制备海藻酸盐膜。制备了两种不同银浓度的薄膜。它们表现出良好的水合性质和非常缓慢的银释放。薄膜对金黄色葡萄球菌和铜绿假单胞菌表现出抗菌和抗生物膜活性，对人皮肤角质形成细胞和人纤维母细胞 HuDe 无细胞毒性。
