Key Laboratory of UV-Emitting Materials and Technology (Northeast Normal University), Ministry of Education, Changchun, Jilin 130024, China.
School of Life Sciences, Northeast Normal University, Changchun, Jilin 130024, China.
Mater Sci Eng C Mater Biol Appl. 2020 Jul;112:110941. doi: 10.1016/j.msec.2020.110941. Epub 2020 Apr 7.
Pectin nanofiber mats are promising tissue engineering scaffolds but suffer from poor cell infiltration. In this study, gelatin, a collagen derived cell adhesive protein, was used to crosslink the electrospun nanofibers of periodate oxidized pectin. Cell culture experiment results demonstrated that cells were able to grow into the gelatin-crosslinked pectin nanofiber mats rather than only spread on mat surface. The nanofiber mats showed moderate mechanical strength, with a maximum tensile strength of up to 2.3 MPa, an ultimate tensile strain of up to 15%, and were capable of degrading gradually over 4 weeks or even longer periods in simulated body fluids. Thus, gelatin-crosslinked pectin nanofiber mats hold a great potential for soft tissue regeneration.
果胶纳米纤维垫是很有前途的组织工程支架，但细胞渗透能力较差。在这项研究中，明胶，一种胶原蛋白来源的细胞黏附蛋白，被用来交联过碘酸钠氧化的果胶的电纺纳米纤维。细胞培养实验结果表明，细胞能够长入明胶交联的果胶纳米纤维垫内，而不仅仅是在垫表面铺展。纳米纤维垫具有适中的机械强度，最大拉伸强度高达 2.3 MPa，最大拉伸应变为 15%，并能在模拟体液中逐渐降解 4 周甚至更长时间。因此，明胶交联的果胶纳米纤维垫在软组织再生方面具有巨大的潜力。
