Wang Yiru, Yan Ying, Yang Mengying, Yang Zhijun
1Department of Gynaecology, Guangxi Medical University Affiliated Tumor Hospital, No. 71 Hedi Road, Nanning, 530021 China.
2Key Laboratory of Early Prevention and Treatment for Regional High Frequency Tumor, Ministry of Education, Guangxi Medical University, Nanning, 530021 China.
3 Biotech. 2020 Jun;10(6):246. doi: 10.1007/s13205-020-02237-x. Epub 2020 May 11.
This study aimed to explore the expressions of signal transducer and activator of transcription 3 (STAT3) and a gene associated with retinoid-interferon induced mortality (Grim19) in epithelial ovarian cancer (EOC), and to determine their correlations with tumor progression and metastasis as well as the related mechanism. Ovarian tissue specimens resected through operation in our hospital were collected, and the correlations of Grim19 and STAT3 expressions with clinicopathological indexes were detected via immunohistochemistry (IHC) and Western blotting. Their positions in cells were observed through immunofluorescence. IHC assay results showed that STAT3 had the lowest expression level in the normal ovary, followed by those in benign ovarian tumor and borderline ovarian tumor (BOT), but it had high expression in EOC; The expression level of Grim19 was the lowest in EOC, followed by those in BOT and benign ovarian tumor successively, while it was highly expressed in the normal ovary; The expressions of STAT3 and Grim19 presented negative correlations in all kinds of ovarian tissues ( < 0.05). The expression level of STAT3 in EOC had no obvious correlations with FIGO staging or WHO classification ( > 0.05). The expression level of Grim19 in EOC in stage FIGO III-IV was higher than that in stage FIGO I-II ( < 0.05), Grim19 expression was not obviously associated with WHO classification ( > 0.05). The expressions of Grim19 and STAT3 in lymphatic metastasis lesion had significantly positive correlations with the primary lesion ( < 0.05). The Western blotting assay results were identical with the IHC results. The immunofluorescence demonstrated that STAT3 and Grim19 were mainly localized in the cytoplasm and they were colocalized in mitochondria. In conclusion, STAT3 presents high expression in EOC tissues while Grim19 is expressed in EOC tissues at a low level, which may be related to its interaction with STAT3 as well as progression, metastasis and poor prognosis of ovarian cancer.
本研究旨在探讨信号转导与转录激活因子3（STAT3）和类视黄醇-干扰素诱导死亡率相关基因（Grim19）在上皮性卵巢癌（EOC）中的表达情况，并确定它们与肿瘤进展、转移的相关性及相关机制。收集我院手术切除的卵巢组织标本，通过免疫组织化学（IHC）和蛋白质免疫印迹法检测Grim19和STAT3表达与临床病理指标的相关性。通过免疫荧光观察它们在细胞中的定位。IHC检测结果显示，STAT3在正常卵巢中的表达水平最低，其次是良性卵巢肿瘤和卵巢交界性肿瘤（BOT），而在EOC中呈高表达；Grim19的表达水平在EOC中最低，其次依次为BOT和良性卵巢肿瘤，而在正常卵巢中高表达；STAT3和Grim19在各类卵巢组织中的表达呈负相关（<0.05）。EOC中STAT3的表达水平与国际妇产科联盟（FIGO）分期或世界卫生组织（WHO）分类无明显相关性（>0.05）。FIGO III-IV期EOC中Grim19的表达水平高于FIGO I-II期（<0.05），Grim19表达与WHO分类无明显相关性（>0.05）。Grim19和STAT3在淋巴转移灶中的表达与原发灶呈显著正相关（<0.05）。蛋白质免疫印迹法检测结果与IHC结果一致。免疫荧光显示，STAT3和Grim19主要定位于细胞质，且在线粒体中共定位。综上所述，STAT3在EOC组织中呈高表达，而Grim19在EOC组织中低表达，这可能与其与STAT3的相互作用以及卵巢癌的进展、转移和预后不良有关。
