National Maize Improvement Center of China, Key Laboratory of Crop Heterosis and Ultilization/Engineering Research Center for Maize Breeding, Ministry of Education, College of Agronomy and Biotechnology, China Agricultural University, Beijing, China.
Bioscience, Wageningen University and Research, Wageningen, the Netherlands.
Nat Plants. 2020 May;6(5):466-472. doi: 10.1038/s41477-020-0658-7. Epub 2020 May 15.
Doubled haploid technology using inducer lines carrying mutations in ZmPLA1/MTL/NLD and ZmDMP has revolutionized traditional maize breeding. ZmPLA1/MTL/NLD is conserved in monocots and has been used to extend the system from maize to other monocots, but no functional orthologue has been identified in dicots, while ZmDMP-like genes exist in both monocots and dicots. Here, we report that loss-of-function mutations in the Arabidopsis thaliana ZmDMP-like genes AtDMP8 and AtDMP9 induce maternal haploids, with an average haploid induction rate of 2.1 ± 1.1%. In addition, to facilitate haploid seed identification in dicots, we established an efficient FAST-Red fluorescent marker-based haploid identification system that enables the identification of haploid seeds with >90% accuracy. These results show that mutations in DMP genes also trigger haploid induction in dicots. The conserved expression patterns and amino acid sequences of ZmDMP-like genes in dicots suggest that DMP mutations could be used to develop in vivo haploid induction systems in dicots.
利用携带ZmPLA1/MTL/NLD 和ZmDMP 突变的诱导系的双倍单倍体技术彻底改变了传统的玉米育种。ZmPLA1/MTL/NLD 在单子叶植物中保守，已被用于将该系统从玉米扩展到其他单子叶植物，但在双子叶植物中未鉴定出功能同源物，而ZmDMP 样基因存在于单子叶植物和双子叶植物中。在这里，我们报告拟南芥ZmDMP 样基因AtDMP8 和 AtDMP9 的功能丧失突变诱导母系单倍体，平均单倍体诱导率为 2.1±1.1%。此外，为了促进双子叶植物中单倍体种子的鉴定，我们建立了一种基于 FAST-Red 荧光标记的高效单倍体鉴定系统，可实现>90%准确率的单倍体种子鉴定。这些结果表明，DMP 基因的突变也会在双子叶植物中引发单倍体诱导。DMP 样基因在双子叶植物中的保守表达模式和氨基酸序列表明，DMP 突变可用于开发双子叶植物体内单倍体诱导系统。
