Nasution Rizha Anshori, Islam Andi Asadul, Hatta Mochammad
Department of Neurosurgery, Pelamonia Hospital, Makassar, Indonesia.
Doctoral Program of Medical Sciences, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia.
Ann Med Surg (Lond). 2020 May 4;54:89-92. doi: 10.1016/j.amsu.2020.04.015. eCollection 2020 Jun.
Peripheral leukocytes can worsen brain damage due to the release of cytotoxic mediators that interfere the blood brain barrier function. One of the oxidants released by activation leukocyte is hypochlorite acid (HOCl) which is formed through the myeloperoxidase (MPO)-H2O2-Cl system. The neuroprotective effects of an experimental anti-inflammatory drug Caffeic Acid Phenethyl Ester (CAPE) tested in a Traumatic brain injury (TBI) model using Myeloperoxidase (MPO) analysis.
This study compares the acute inflammatory response to TBI over time, as measured by MPO activity. Adult Sprague mice were treated for head trauma with . At 24 h before trauma, all animals were blood test (n = 10) to examine MPO, then the animal was divided into 2 groups of injured animals treated with CAPE (n = 5), and those not treated with CAPE (n = 5). We used the MPO test to identify the level of polymorphonuclear leukocytes (PMNL) on day 4 and day 7.
Showed an increase in PMNL infiltration in CAPE untreated animals, this change significantly (P < 0.05) decreased at group of animals treated with CAPE. MPO serum activity in the CAPE untreated group vs treated with CAPE on day 4 ± 11920410.076 (Standard deviation {SD} 895355.169) vs 6663184.485 (SD 895355.169) p < 0,05 and on day 7 ± 14223286.992 (SD 802762.401) vs 9284222.028 (SD 953098.093) p < 0,05. These data indicate that MPO activity after TBI increases on day 4 also on day 7 and improves after being treated with CAPE.
CAPE can reduce Neutrophil serum levels there by preventing brain damage in TBI.
外周白细胞可因释放干扰血脑屏障功能的细胞毒性介质而加重脑损伤。活化白细胞释放的一种氧化剂是次氯酸（HOCl），它通过髓过氧化物酶（MPO）-H2O2-Cl系统形成。使用髓过氧化物酶（MPO）分析在创伤性脑损伤（TBI）模型中测试了一种实验性抗炎药物咖啡酸苯乙酯（CAPE）的神经保护作用。
本研究通过MPO活性比较了随时间推移对TBI的急性炎症反应。成年Sprague小鼠接受头部创伤治疗。在创伤前24小时，对所有动物进行血液检测（n = 10）以检测MPO，然后将动物分为两组，一组为接受CAPE治疗的受伤动物（n = 5），另一组为未接受CAPE治疗的动物（n = 5）。我们使用MPO测试来确定第4天和第7天多形核白细胞（PMNL）的水平。
结果显示，未接受CAPE治疗的动物中PMNL浸润增加，而在接受CAPE治疗的动物组中，这种变化显著降低（P < 0.05）。在第4天，未接受CAPE治疗组的MPO血清活性±11920410.076（标准差{SD} 895355.169），而接受CAPE治疗组为6663184.485（SD 895355.169），p < 0.05；在第7天，未接受CAPE治疗组的MPO血清活性±14223286.992（SD 802762.401），而接受CAPE治疗组为9284222.028（SD 953098.093），p < 0.05。这些数据表明，TBI后第4天和第7天MPO活性增加，而在接受CAPE治疗后有所改善。
CAPE可降低中性粒细胞血清水平，从而预防TBI中的脑损伤。
