Tommy Thomas, Islam Andi A, Hatta Mochammad, Bukhari Agussalim, Adhimarta Willy, Zainuddin Andi Alfian
Department of Neurosurgery, Faculty of Medicine, Universitas Pelita Harapan, Tangerang, Indonesia.
Department of Neurosurgery, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia.
Ann Med Surg (Lond). 2021 Apr 6;65:102273. doi: 10.1016/j.amsu.2021.102273. eCollection 2021 May.
Head injury or traumatic brain injury is the leading cause of mortality and morbidity. Many modalities of neuroprotection had been developed in brain injury but there was no much information regarding folinic acid's effect on neuroinflammation associated with homocysteine, TNFα, IL-10, and HMGB1.
This study aimed to investigate whether folinic acid has improving effect on head injury model.
This study was done in the rat's head injury model using modified Marmarou weight drop model. Fifteen rats were randomized and grouped into 3 groups: Group A: Folinic acid (+), head injury (-); Group B: Folinic acid (-), head injury (+); Group C: Folinic acid (+), head injury (+). Folinic acid was administered intraperitoneally with a dose of 60 mg/m. Blood samples were taken immediately after head injury (H0), 12 h (H12), and 24 h (H24) after head injury from the lateral vein of tail. Serum level of homocysteine, TNFα, and IL-10 were measured using ELISA, and HMGB1 gene expression was measured with Real-Time RT-PCR.
This study found serum level of homocysteine, TNFα, IL-10 and HMGB1 gene expression were markedly increased at all time points after head injury. Significantly lower level of serum homocysteine, TNFα, IL-10 and HMGB1 gene expression were found after 24 h treatment with folinic acid in group C compared to those in group B.
Folinic acid may have anti-inflammatory properties in traumatic brain injury by inhibition of serum level of homocysteine, TNFα, IL-10 and HMGB1 gene expression.
头部损伤或创伤性脑损伤是导致死亡和发病的主要原因。在脑损伤中已开发出多种神经保护方法，但关于亚叶酸对与同型半胱氨酸、肿瘤坏死因子α（TNFα）、白细胞介素-10（IL-10）和高迁移率族蛋白B1（HMGB1）相关的神经炎症的影响，目前所知甚少。
本研究旨在探讨亚叶酸对头部损伤模型是否具有改善作用。
本研究采用改良的 Marmarou 重物坠落模型建立大鼠头部损伤模型。将 15 只大鼠随机分为 3 组：A 组：亚叶酸（+），头部损伤（-）；B 组：亚叶酸（-），头部损伤（+）；C 组：亚叶酸（+），头部损伤（+）。亚叶酸以 60mg/m 的剂量腹腔注射。在头部损伤后即刻（H0）、损伤后 12 小时（H12）和 24 小时（H24）从尾侧静脉采集血样。使用酶联免疫吸附测定法（ELISA）检测同型半胱氨酸、TNFα和 IL-10 的血清水平，并用实时逆转录聚合酶链反应（Real-Time RT-PCR）检测 HMGB1 基因表达。
本研究发现，头部损伤后所有时间点同型半胱氨酸、TNFα、IL-10 的血清水平和 HMGB1 基因表达均显著升高。与 B 组相比，C 组在接受亚叶酸治疗 24 小时后，血清同型半胱氨酸、TNFα、IL-10 水平和 HMGB1 基因表达明显降低。
亚叶酸可能通过抑制同型半胱氨酸、TNFα、IL-10 的血清水平和 HMGB1 基因表达，在创伤性脑损伤中具有抗炎特性。
