弹性组织用于可逆形状变换和加速分子标记。
Elasticizing tissues for reversible shape transformation and accelerated molecular labeling.
机构信息
Institute for Medical Engineering and Science, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA.
Picower Institute for Learning and Memory, MIT, Cambridge, MA, USA.
出版信息
Nat Methods. 2020 Jun;17(6):609-613. doi: 10.1038/s41592-020-0823-y. Epub 2020 May 18.
Abstract
We developed entangled link-augmented stretchable tissue-hydrogel (ELAST), a technology that transforms tissues into elastic hydrogels to enhance macromolecular accessibility and mechanical stability simultaneously. ELASTicized tissues are highly stretchable and compressible, which enables reversible shape transformation and faster delivery of probes into intact tissue specimens via mechanical thinning. This universal platform may facilitate rapid and scalable molecular phenotyping of large-scale biological systems, such as human organs.
摘要
我们研发了纠缠链接增强可拉伸组织水凝胶（ELAST）技术，该技术可将组织转化为弹性水凝胶，同时提高大分子的可及性和机械稳定性。ELASTicized 组织具有高拉伸性和可压缩性，可实现形状的可逆转变，并通过机械变薄将探针更快地递送至完整的组织标本中。这个通用平台可能有助于快速和规模化地对大规模生物系统（如人体器官）进行分子表型分析。
相似文献
1
Elasticizing tissues for reversible shape transformation and accelerated molecular labeling.弹性组织用于可逆形状变换和加速分子标记。
Nat Methods. 2020 Jun;17(6):609-613. doi: 10.1038/s41592-020-0823-y. Epub 2020 May 18.
2
Tunable mechanical stability and deformation response of a resilin-based elastomer.基于橡胶蛋白的弹性体的可调机械稳定性和变形响应。
Biomacromolecules. 2011 Jun 13;12(6):2302-10. doi: 10.1021/bm200373p. Epub 2011 May 25.
3
Material properties in unconfined compression of gelatin hydrogel for skin tissue engineering applications.用于皮肤组织工程应用的明胶水凝胶无侧限压缩中的材料特性。
Biomed Tech (Berl). 2014 Dec;59(6):479-86. doi: 10.1515/bmt-2014-0028.
4
Influence of physical and mechanical properties of amphiphilic biosynthetic hydrogels on long-term cell viability.两亲性生物合成水凝胶的物理和机械性能对细胞长期活力的影响。
J Mech Behav Biomed Mater. 2014 Jul;35:111-22. doi: 10.1016/j.jmbbm.2014.03.010. Epub 2014 Apr 12.
5
Role of temperature on bio-printability of gelatin methacryloyl bioink in two-step cross-linking strategy for tissue engineering applications.温度在用于组织工程应用的两步交联策略中对甲基丙烯酰化明胶生物墨水生物可打印性的作用。
Biomed Mater. 2020 Dec 16;16(1):015021. doi: 10.1088/1748-605X/abbcc9.
6
Examining the Impact of Squaric Acid as a Crosslinking Agent on the Properties of Chitosan-Based Films.研究丁烯二酸作为交联剂对壳聚糖基薄膜性能的影响。
Int J Mol Sci. 2021 Mar 24;22(7):3329. doi: 10.3390/ijms22073329.
7
Reinforcing interpenetrating network hydrogels with 3D printed polymer networks to engineer cartilage mimetic composites.通过 3D 打印聚合物网络增强互穿网络水凝胶，以工程软骨模拟复合材料。
Biofabrication. 2020 May 12;12(3):035011. doi: 10.1088/1758-5090/ab8708.
8
Soft network composite materials with deterministic and bio-inspired designs.具有确定性和仿生设计的软网络复合材料。
Nat Commun. 2015 Mar 18;6:6566. doi: 10.1038/ncomms7566.
9
3D bioprinting of urethra with PCL/PLCL blend and dual autologous cells in fibrin hydrogel: An in vitro evaluation of biomimetic mechanical property and cell growth environment.聚己内酯/聚己内酯-聚乳酸共聚物共混物与双自体细胞在纤维蛋白水凝胶中进行尿道的3D生物打印：仿生力学性能和细胞生长环境的体外评估
Acta Biomater. 2017 Mar 1;50:154-164. doi: 10.1016/j.actbio.2016.12.008. Epub 2016 Dec 8.
10
Advances in bioactive hydrogels to probe and direct cell fate.生物活性水凝胶在探测和指导细胞命运方面的研究进展。
Annu Rev Chem Biomol Eng. 2012;3:421-44. doi: 10.1146/annurev-chembioeng-062011-080945. Epub 2012 Apr 17.
引用本文的文献
1
Advances in tissue optical clearing for 3D imaging in large animal.大型动物三维成像的组织光学透明技术进展
Front Optoelectron. 2025 Aug 18;18(1):18. doi: 10.1007/s12200-025-00162-6.
2
ht-MASH: a high-throughput, cost-effective, and robust protocol for microscopic 3D imaging of human angio- and cytoarchitecture in large human brain samples.ht-MASH：一种用于对大型人脑样本中的人类血管和细胞结构进行微观三维成像的高通量、经济高效且稳健的方案。
Anat Sci Int. 2025 Jul 8. doi: 10.1007/s12565-025-00859-w.
3
Critical impact of lysine 136 in TDP-43 phase separation, compartmentalization, and aggregation in living vertebrates.赖氨酸136对活脊椎动物中TDP-43相分离、区室化和聚集的关键影响。
iScience. 2025 May 27;28(7):112761. doi: 10.1016/j.isci.2025.112761. eCollection 2025 Jul 18.
4
A three dimensional immunolabeling method with peroxidase-fused nanobodies and fluorochromized tyramide-glucose oxidase signal amplification.一种使用过氧化物酶融合纳米抗体和荧光素化酪胺-葡萄糖氧化酶信号放大的三维免疫标记方法。
Commun Biol. 2025 Jun 18;8(1):903. doi: 10.1038/s42003-025-08317-z.
5
Three-dimensional assessments are necessary to determine the true, spatially resolved composition of tissues.三维评估对于确定组织的真实、空间分辨组成是必要的。
Cell Rep Methods. 2025 Jun 16;5(6):101075. doi: 10.1016/j.crmeth.2025.101075. Epub 2025 Jun 9.
6
Increased CSF drainage by non-invasive manipulation of cervical lymphatics.通过对颈部淋巴管进行非侵入性操作增加脑脊液引流。
Nature. 2025 Jun 4. doi: 10.1038/s41586-025-09052-5.
7
Uniform volumetric single-cell processing for organ-scale molecular phenotyping.用于器官尺度分子表型分析的均匀体积单细胞处理
Nat Biotechnol. 2025 Jan 24. doi: 10.1038/s41587-024-02533-4.
8
INSIHGT: an accessible multi-scale, multi-modal 3D spatial biology platform.INSIHGT：一个易于使用的多尺度、多模态3D空间生物学平台。
Nat Commun. 2024 Dec 30;15(1):10888. doi: 10.1038/s41467-024-55248-0.
9
SHARD: an improved method for staining and visualizing multiplex immunofluorescence in optically cleared postmortem human brain tissue.SHARD：一种用于在光学透明的死后人类脑组织中对多重免疫荧光进行染色和可视化的改进方法。
Front Neurosci. 2024 Oct 9;18:1474617. doi: 10.3389/fnins.2024.1474617. eCollection 2024.
10
FLUID: a fluorescence-friendly lipid-compatible ultrafast clearing method.FLUID：一种对荧光友好的脂质兼容超快速清除方法。
Biomed Opt Express. 2024 Sep 3;15(10):5609-5624. doi: 10.1364/BOE.533072. eCollection 2024 Oct 1.
本文引用的文献
1
Publisher Correction: Tissue clearing and its applications in neuroscience.出版商更正：组织透明化及其在神经科学中的应用。
Nat Rev Neurosci. 2020 May;21(5):298. doi: 10.1038/s41583-020-0291-5.
2
Cellular and Molecular Probing of Intact Human Organs.完整人体器官的细胞和分子探测。
Cell. 2020 Feb 20;180(4):796-812.e19. doi: 10.1016/j.cell.2020.01.030. Epub 2020 Feb 13.
3
Scalable and Isotropic Expansion of Tissues with Simply Tunable Expansion Ratio.具有简单可调扩张比的组织的可扩展各向同性扩张
Adv Sci (Weinh). 2019 Sep 30;6(22):1901673. doi: 10.1002/advs.201901673. eCollection 2019 Nov.
4
Protection of tissue physicochemical properties using polyfunctional crosslinkers.使用多功能交联剂保护组织物理化学性质
Nat Biotechnol. 2018 Dec 17. doi: 10.1038/nbt.4281.
5
Three-dimensional intact-tissue sequencing of single-cell transcriptional states.单细胞转录状态的三维完整组织测序。
Science. 2018 Jul 27;361(6400). doi: 10.1126/science.aat5691. Epub 2018 Jun 21.
6
A three-dimensional single-cell-resolution whole-brain atlas using CUBIC-X expansion microscopy and tissue clearing.采用 CUBIC-X 扩展显微镜和组织透明化技术的三维单细胞分辨率全脑图谱
Nat Neurosci. 2018 Apr;21(4):625-637. doi: 10.1038/s41593-018-0109-1. Epub 2018 Mar 5.
7
Whole-Body Profiling of Cancer Metastasis with Single-Cell Resolution.以单细胞分辨率进行癌症转移的全身分析
Cell Rep. 2017 Jul 5;20(1):236-250. doi: 10.1016/j.celrep.2017.06.010.
8
Advances in engineering hydrogels.工程水凝胶的进展
Science. 2017 May 5;356(6337). doi: 10.1126/science.aaf3627.
9
MeCP2-regulated miRNAs control early human neurogenesis through differential effects on ERK and AKT signaling.MeCP2 调控的 microRNAs 通过对 ERK 和 AKT 信号通路的不同影响来控制早期人类神经发生。
Mol Psychiatry. 2018 Apr;23(4):1051-1065. doi: 10.1038/mp.2017.86. Epub 2017 Apr 25.
10
Quantifying the impact of molecular defects on polymer network elasticity.量化分子缺陷对聚合物网络弹性的影响。
Science. 2016 Sep 16;353(6305):1264-8. doi: 10.1126/science.aag0184.
Zotero 插件