Department of Clinical and Experimental Medicine, University of Foggia, Foggia, Italy.
Department of Biophysical Chemistry, Heidelberg University & Max Planck Institute for Medical Research, Heidelberg, Germany.
Front Endocrinol (Lausanne). 2020 May 5;11:285. doi: 10.3389/fendo.2020.00285. eCollection 2020.
Hard tissue regeneration represents a challenge for the Regenerative Medicine and Mesenchymal stem cells (MSCs) could be a successful therapeutic strategy. T-LysYal® (T-Lys), a new derivative of Hyaluronic Acid (HA) possessing a superior stability, has already been proved efficient in repairing corneal epithelial cells damaged by dry conditions . We investigated the regenerative potential of T-Lys in the hard tissues bone and cartilage. We have previously demonstrated that cells isolated from the tooth germ, Dental Bud Stem Cells (DBSCs), differentiate into osteoblast-like cells, representing a promising source of MSCs for bone regeneration. Herewith, we show that T-Lys treatment stimulates the expression of typical osteoblastic markers, such as () and Alkaline Phosphatase (ALP), determining a higher production of mineralized matrix nodules. In addition, we found that T-Lys treatment positively affects αβ integrin expression, key integrin in the osteoblastic commitment, leading to the formation of focal adhesions (FAs). The efficacy of T-Lys was also tested on chondrogenic differentiation starting from human articular chondrocytes (HACs) resulting in an increase of differentiation markers and cell number.
硬组织再生是再生医学的一个挑战，间充质干细胞（MSCs）可能是一种成功的治疗策略。T-LysYal®（T-Lys）是透明质酸（HA）的一种新型衍生物，具有更高的稳定性，已被证明能有效修复因干燥条件而受损的角膜上皮细胞。我们研究了 T-Lys 在硬组织骨和软骨中的再生潜力。我们之前已经证明，从牙胚中分离出来的牙髓干细胞（DBSCs）分化为成骨样细胞，代表了一种有前途的用于骨再生的间充质干细胞来源。在此，我们表明 T-Lys 处理可刺激典型成骨细胞标志物的表达，如骨钙素（OCN）和碱性磷酸酶（ALP），从而导致矿化基质结节的产生增加。此外，我们发现 T-Lys 处理可正向影响αβ整联蛋白的表达，αβ整联蛋白是成骨细胞分化的关键整合素，从而导致焦点黏附（FA）的形成。我们还在从人关节软骨细胞（HAC）开始的软骨分化中测试了 T-Lys 的功效，结果表明分化标志物和细胞数量增加。
