球虫作为糠秕马拉色菌和厚皮马拉色菌感染模型的标准化。

Standardization of Galleria mellonella as an Infection Model for Malassezia furfur and Malassezia pachydermatis.

机构信息

Grupo de Investigación Celular y Molecular de Microorganismos Patógenos (CeMoP), Departamento de Ciencias Biológicas, Universidad de los Andes, Bogotá, Colombia.

出版信息

Methods Mol Biol. 2023;2667:15-29. doi: 10.1007/978-1-0716-3199-7_2.

DOI:10.1007/978-1-0716-3199-7_2

PMID:37145273

Abstract

Galleria mellonella larva has been widely exploited as an infection model for bacteria and fungi. Our laboratory uses this insect as a model for fungal infection caused by the genus Malassezia, in particular, systemic infections caused by Malassezia furfur and Malassezia pachydermatis, which are poorly understood. Here, we describe the G. mellonella larva inoculation process with M. furfur and M. pachydermatis and the posterior assessment of the establishment and dissemination of the infection in the larvae. This assessment was done through the evaluation of larval survival, melanization, fungal burden, hemocytes populations, and histological changes. This methodology allows for the identification of virulence patterns between Malassezia species and the impact of inoculum concentration and temperature.

摘要

家蚕幼虫已被广泛用作细菌和真菌感染模型。我们实验室使用这种昆虫作为马拉色菌属真菌感染的模型，特别是对糠秕马拉色菌和厚皮马拉色菌引起的系统性感染知之甚少。在这里，我们描述了用糠秕马拉色菌和厚皮马拉色菌对家蚕幼虫进行接种的过程，以及对幼虫感染建立和传播的后续评估。通过评估幼虫的存活率、黑化、真菌负荷、血细胞群和组织学变化来进行评估。这种方法可以鉴定出马色菌属种之间的毒力模式，以及接种物浓度和温度的影响。