College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Qingdao University, Qingdao 266071, China.
College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China.
ACS Appl Mater Interfaces. 2020 Jun 10;12(23):25565-25571. doi: 10.1021/acsami.0c04317. Epub 2020 Jun 1.
The encapsulation of multiple enzyme/nanoenzyme systems within mental-organic frameworks (MOFs) shows great promise for a myriad of practical applications. Herein, two sequential biocatalysts, oxidase and hemin, were coupled together with close proximity using a bifunctional polymer, poly(1-vinylimidazole) (PVI), and encapsulated into MOFs. As a demonstration of the power of such a protocol, glucose oxidase&PVI-hemin encapsulated in ZIF-8 showed significant enhancement of bioactivity for a cascade reaction compared to its counterpart without PVI. For the colorimetric assay of glucose, it showed a low limit of detection of 0.4 μM (S/N = 3), high selectivity, and excellent stability. Because there are numerous biocatalysts that can readily be coupled and encapsulated into MOFs, a myriad of interesting properties can be simply realized by encapsulating different sequential biocatalysts.
将多种酶/纳米酶系统封装在金属有机框架（MOFs）内，为许多实际应用展现了巨大的应用前景。在此，使用双功能聚合物聚（1-乙烯基咪唑）（PVI）将两种连续的生物催化剂氧化酶和血红素紧密地偶联在一起，并封装到 MOFs 中。作为这种方案的强大功能的一个演示，与没有 PVI 的对应物相比，封装在 ZIF-8 中的葡萄糖氧化酶&PVI-血红素的级联反应的生物活性显著增强。对于葡萄糖的比色测定，它显示出低至 0.4 μM（S/N = 3）的检测限、高选择性和优异的稳定性。由于有许多生物催化剂可以很容易地偶联并封装到 MOFs 中，因此通过封装不同的连续生物催化剂可以简单地实现多种有趣的性质。
