Nematology Institute of Northern China, Shenyang Agricultural University, Shenyang, 110866, P.R. China.
College of Biosciences and Biotechnology, Shenyang Agricultural University, Shenyang, 110866, P.R. China.
Sci Rep. 2020 May 20;10(1):8342. doi: 10.1038/s41598-020-65262-z.
Root-knot nematode (Meloidogyne incognita) is chief plant parasitic nematode of various crops globally. Meanwhile, the negative side effects on human health and environmental concerns associated with haphazard uses of chemical nematicides. Hence, the search for a safe and effective approach is more relevant. The present study was aimed to evaluate the nematicidal potential of Snef1216 (Penicillium chrysogenum) against M. incognita at different concentrations (5%, 10%, 25%, 50%, 75% and 100%) and with the nutritious medium. The egg hatching inhibition and mortality of second stage juveniles of M. incognita were assessed after 6, 12, 24, 48 and 72 h exposure. Results revealed that egg hatching inhibition and percent mortality of M. incognita increased with increasing concentration and exposure time. The highest mortality of juveniles was recorded at 100% conc. i.e., 24.20%, 36%, 66%, 78% and 97.8% at 6, 12, 24, 48 and 72 h, respectively. The highest ovicidal activity was recorded at 100% concentration with 5.20% of eggs hatching. The outcome suggested that Snef1216 (P. chrysogenum) resulted in the lowest LC value was recorded as 3718.496 at 6 h exposure period followed by 10479.87, 11186.682, 14838.58 and 24001.430 at 72, 12, 48 and 24 h respectively via ovicidal assay. Whereas, in the larvicidal assay, the lowest LC value demonstrated at 72 h being 17.628% exposure period followed by 28.345, 50.490, 215.710 and 482.595% at 48, 24, 12 and 6 h respectively. It is concluded that Snef1216 has potential being used as a biocontrol agent against M. incognita and can serve as a source of a novel nematicidal agent of fungal origin.
根结线虫（Meloidogyne incognita）是全球各种作物的主要植物寄生线虫。同时，由于化学杀线虫剂的随意使用对人类健康和环境产生的负面影响，因此寻找一种安全有效的方法变得更加重要。本研究旨在评估 Snef1216（青霉素）在不同浓度（5%、10%、25%、50%、75%和 100%）和营养培养基中对 M. incognita 的杀线虫潜力。在暴露 6、12、24、48 和 72 小时后，评估 M. incognita 的卵孵化抑制和第二代幼虫死亡率。结果表明，卵孵化抑制和 M. incognita 的死亡率随着浓度和暴露时间的增加而增加。在 100%浓度下记录到最高的幼虫死亡率，即 6、12、24、48 和 72 小时分别为 24.20%、36%、66%、78%和 97.8%。在 100%浓度下记录到最高的杀卵活性，孵化的卵为 5.20%。结果表明，Snef1216（青霉素）导致的最低 LC 值出现在 6 小时暴露期，为 3718.496，随后分别为 10479.87、11186.682、14838.58 和 24001.430，在 72、12、48 和 24 小时的杀卵试验中。而在幼虫杀灭试验中，最低 LC 值出现在 72 小时暴露期，为 17.628%，随后分别为 28.345%、50.490%、215.710%和 482.595%，在 48、24、12 和 6 小时的试验中。综上所述，Snef1216 具有作为防治 M. incognita 的生物防治剂的潜力，可以作为真菌来源的新型杀线虫剂的来源。
