Chinese Medicine School, Beijing University of Chinese Medicine, Beijing 100029, P. R. China.
Am J Chin Med. 2020;48(4):923-944. doi: 10.1142/S0192415X20500445. Epub 2020 May 21.
Chronic insomnia is a disease which brings intense mental pain and disturbing complications to patients worldwide. The oral microbiome exhibits a mechanistic influence on human health. Therefore, it is crucial to understand the oral microbial diversity in insomnia. Tongue diagnosis has been considered a critical basic procedure in insomnia therapeutic decision-making in Traditional Chinese Medicine (TCM). Hence, it is significant to elucidate the various oral microbiome differences in chronic insomnia patients with different tongue features. In this paper, we used 16S rRNA gene sequencing and bioinformatics analysis to investigate dynamic changes in oral bacterial profile and correlations between chronic insomnia patients and healthy individuals, as well as in patients with different tongue coatings. Moreover, the relationship between the severity of insomnia and oral microbiota was explored. Our findings showed that chronic insomnia patients harbored a significantly higher diversity of oral bacteria when compared to healthy controls. More importantly, the results revealed that the diversity and relative abundance of the bacterial community was significantly altered among different tongue coatings in patients but not in healthy individuals. Oral bacteria with a relative abundance [Formula: see text]1% and [Formula: see text] among different tongue groups were considered remarkable bacteria, which included three phyla and four genera, , and . Our findings indicate that changes in oral microbiome correlate with tongue coatings in patients with chronic insomnia. Thus, the remarkable microbiome may provide inspiration for further studies on the correlation between tongue diagnosis and oral microbiome in chronic insomnia patients.
慢性失眠是一种全球性疾病，给患者带来强烈的精神痛苦和困扰。口腔微生物组对人类健康具有机制影响。因此，了解失眠患者的口腔微生物多样性至关重要。舌诊被认为是中医治疗失眠决策的关键基本程序。因此，阐明具有不同舌象特征的慢性失眠患者口腔微生物组的各种差异具有重要意义。在本文中，我们使用 16S rRNA 基因测序和生物信息学分析来研究慢性失眠患者与健康个体以及不同舌苔患者之间口腔细菌谱的动态变化以及它们之间的相关性。此外，还探讨了失眠严重程度与口腔微生物群之间的关系。我们的研究结果表明，与健康对照组相比，慢性失眠患者的口腔细菌多样性明显更高。更重要的是，结果表明，不同舌苔患者的细菌群落多样性和相对丰度发生了显著变化，但健康个体则没有。在不同舌象组中相对丰度为 [Formula: see text]1%和 [Formula: see text]的口腔细菌被认为是显著细菌，包括三个门和四个属 、 、 和 。我们的研究结果表明，口腔微生物组的变化与慢性失眠患者的舌苔有关。因此，显著的微生物组可能为进一步研究慢性失眠患者舌诊与口腔微生物组之间的相关性提供启示。
