Instituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Edifico B3, Ciudad Universitaria, C. P. 58030, Morelia, Michoacán, México.
Department of Biotechnology and Biochemistry, Cinvestav Unidad Irapuato. Km. 9.6 Libramiento Norte Carr. Irapuato-León. C. P. 36824, Irapuato, Guanajuato, México.
Sci Rep. 2020 May 21;10(1):8426. doi: 10.1038/s41598-020-65314-4.
Biofertilizer production and application for sustainable agriculture is already a reality. The methods for biofertilizers delivery in crop fields are diverse. Although foliar spray is gaining wide acceptance, little is known about the influence that the biochemical features of leaves have on the microbial colonization. Arthrobacter agilis UMCV2 is a rhizospheric and endophytic bacteria that promotes plant growth and health. In this study, we determined the capacity of the UMCV2 strain to colonize different leaves from Medicago truncatula in a foliar inoculation system. By using two powerful analytical methods based on mass spectrometry, we determined the chemical profile of the leaves in 15-d old plants. The metabolic signatures between the unifoliate leaf (m1) and the metameric units developing above (m2 and m3) were different, and interestingly, the highest colony forming units (CFU) was found in m1. The occurrence of the endophyte strongly affects the sugar composition in m1 and m2 leaves. Our results suggest that A. agilis UMCV2 colonize the leaves under a foliar inoculation system independently of the phenological age of the leaf and it is capable of modulating the carbohydrate metabolism without affecting the rest of the metabolome.
生物肥料的生产和应用已经成为可持续农业的现实。生物肥料在农田中的施用方法多种多样。虽然叶面喷施已经得到广泛认可，但对于叶片的生化特性对微生物定殖的影响却知之甚少。节杆菌 UMCV2 是一种根际和内生细菌，能促进植物的生长和健康。在这项研究中，我们确定了 UMCV2 菌株在叶面接种系统中定殖不同紫花苜蓿叶片的能力。我们使用两种基于质谱的强大分析方法，确定了 15 天龄植物叶片的化学特征。单叶（m1）和上方分生组织（m2 和 m3）之间的代谢特征不同，有趣的是，m1 中发现的菌落形成单位（CFU）最高。内生菌的出现强烈影响 m1 和 m2 叶片中的糖组成。我们的结果表明，A. agilis UMCV2 在叶面接种系统中定殖叶片，独立于叶片的物候年龄，并且能够调节碳水化合物代谢，而不影响其余的代谢组。
