Gebremeskel Leake, Beshir Tuem Kald, Teklu Tewolde
Pharmacology and Toxicology Unit, Department of Pharmacy, College of Health Sciences, Aksum University, Aksum, Ethiopia.
Department of Pharmacology and Toxicology, School of Pharmacy, College of Health Sciences, Mekelle University, Mekelle, Ethiopia.
Diabetes Metab Syndr Obes. 2020 May 4;13:1481-1489. doi: 10.2147/DMSO.S246996. eCollection 2020.
has been used traditionally for treatment of different ailments including diabetes mellitus although it lacks scientific evidence. Thus, the present study was aimed at evaluating the antidiabetic effect of in streptozotocin (STZ)-induced diabetic mice.
The antidiabetic activity of hydro-ethanolic (30:70) leaf extract of was evaluated in STZ (45 mg/kg)-induced diabetic and normal mice. Antihyperglycemic, hypoglycemic, oral glucose tolerance and body weight change effects of the extract were assessed after administering three doses of the extract (200, 400 and 600 mg/kg), glibenclamide 5 mg/kg (reference drug) and 2% Tween 80 (vehicle). One-way analysis of variance and Tukey's post hoc test were used for data analysis.
All doses of the extract (200 mg/kg (<0.05), 400 mg/kg (<0.05) and 600 mg/kg (<0.01)) and glibenclamide 5 mg/kg (<0.001) showed statistically significant blood glucose level reduction in normal mice as compared to Tween 80. The hydroalcoholic extract at a dose of 200 mg/kg (<0.05), 400 mg/kg (<0.01) and 600 mg/kg (<0.001) showed better blood glucose tolerance after 60, 120 and 180-minute treatment duration in normal mice as compared to negative control. In diabetic mice, doses and the reference drug caused maximum reduction in blood glucose level at the end of the 15th day of treatment by 17.61%, 22.52%, 24.62% and 34.12%, respectively. The extract's doses and the standard drug showed significant (<0.05) improvement in body weight while the diabetic control continued to lose their body weight.
Thus, exhibits antihyperglycemic activity in STZ-induced diabetic mice, and shows improvement in oral glucose tolerance and body weight, which justifies the claimed use of the plant in ameliorating diabetes mellitus in Ethiopian folk medicine.
尽管缺乏科学依据，但传统上一直被用于治疗包括糖尿病在内的各种疾病。因此，本研究旨在评估其在链脲佐菌素（STZ）诱导的糖尿病小鼠中的抗糖尿病作用。
在STZ（45mg/kg）诱导的糖尿病小鼠和正常小鼠中评估其水乙醇（30:70）叶提取物的抗糖尿病活性。在给予提取物的三个剂量（200、400和600mg/kg）、格列本脲5mg/kg（参比药物）和2%吐温80（赋形剂）后，评估提取物的降血糖、低血糖、口服葡萄糖耐量和体重变化作用。采用单因素方差分析和Tukey事后检验进行数据分析。
与吐温80相比，提取物的所有剂量（200mg/kg（<0.05）、400mg/kg（<0.05）和600mg/kg（<0.01））以及格列本脲5mg/kg（<0.001）在正常小鼠中均显示出统计学上显著的血糖水平降低。与阴性对照相比，200mg/kg（<0.05）、400mg/kg（<0.01）和600mg/kg（<0.001）剂量的水醇提取物在正常小鼠中经过60、120和180分钟的治疗后显示出更好的葡萄糖耐量。在糖尿病小鼠中，提取物的剂量和参比药物在治疗第15天结束时分别使血糖水平最大降低了17.61%、22.52%、24.62%和34.12%。提取物的剂量和标准药物在体重方面显示出显著（<0.05）改善，而糖尿病对照组体重持续减轻。
因此，在STZ诱导的糖尿病小鼠中表现出降血糖活性，并显示出口服葡萄糖耐量和体重的改善，这证明了该植物在埃塞俄比亚民间医学中用于改善糖尿病的说法是合理的。
