Rossen R D, Rickaway R H, Reisberg M A, Singer D B, Schloeder F X, Suki W N, Hill L L, Eknoyan G
Arthritis Rheum. 1977 May;20(4):947-61. doi: 10.1002/art.1780200407.
Localization of fluorescein-conjugated heat-aggregated IgG (FA IgG) was demonstrated by immunofluorescence in renal glomeruli of 16 of 69 patients with glomerulonephritis. FA IgG bound more frequently in kidney biopsies from patients with diffuse glomerulonephritis and depressed renal function, and localized selectively in glomeruli that contained heavy deposits of IgM, C3, and C4. The factors that caused FA IgG to bind were specifically reactive with the Fc piece of the IgG molecule and were resistant to 56 degrees C heat for 30 minutes. Localization of FA IgG in the kidney did not correlate with the presence of soluble immune complexes or detectable antiglobulin antibodies in the sera. Binding of FA IgG was also seen in glomeruli and arteries of 18 of 21 kidney allografts studied at the time of impending rejection. But the factors responsible for binding FA IgG in the allografts were heat labile and thus could have been C1q. Although the role of these "antiglobulins" in the immunobiology of glomerulonephritis remains unknown, the fact that they occurred mainly in patients with relatively severe glomerular injury suggests that they could play some part in promoting renal glomerular injury.
通过免疫荧光法在69例肾小球肾炎患者中的16例患者的肾小球中证实了荧光素结合的热聚集IgG（FA IgG）的定位。FA IgG在弥漫性肾小球肾炎和肾功能减退患者的肾活检中结合更为频繁，并选择性地定位于含有大量IgM、C3和C4沉积物的肾小球中。导致FA IgG结合的因素与IgG分子的Fc片段具有特异性反应，并且在56℃加热30分钟后仍具有抗性。FA IgG在肾脏中的定位与血清中可溶性免疫复合物或可检测到的抗球蛋白抗体的存在无关。在21例即将发生排斥反应时研究的肾移植中的18例的肾小球和动脉中也观察到了FA IgG的结合。但是在同种异体移植物中负责结合FA IgG的因素对热不稳定，因此可能是C1q。尽管这些“抗球蛋白”在肾小球肾炎免疫生物学中的作用尚不清楚，但它们主要发生在相对严重肾小球损伤患者中的事实表明它们可能在促进肾小球损伤中起一定作用。
