Marine College, Shandong University, Weihai, Shandong 264209, PR China.
State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong 266237, PR China.
Int J Syst Evol Microbiol. 2020 Jun;70(6):3588-3596. doi: 10.1099/ijsem.0.004143. Epub 2020 May 26.
A Gram-stain-negative, non-motile, fine rod or short filament shaped, jacinth pigmented bacterium, designated strain WDS2C27, was isolated from a marine solar saltern in Wendeng, Weihai, PR China (37°31'5″ N, 122°1'47″ E). Growth of WDS2C27 occurred at 20-42 °C (optimum 37 °C) and pH 6.5-8.5 (optimal pH 7.0-8.0). Optimal growth occurred in modified marine broth containing 6 % (w/v) NaCl. The major polar lipids in WDS2C27 were phosphatidylethanolamine, two unidentified aminolipids and one unidentified lipid. The major respiratory quinone of WDS2C27 was MK-6. The dominant fatty acids were iso-C and anteisoC. The DNA G+C content was 35.0 mol%. The nucleotide sequence of the 16S rRNA gene indicated that the most closely related strain was X15M-8 (92.0 % over 1452 bp). WDS2C27 showed 60.7 % average amino acid identity, 55.6 % percentage of conserved proteins, 75.0 % average nucleotide identity and 13.1 % digital DNA-DNA hybridization identity with the type species of the genus , ATCC 700755. The phenotypic and genotypic properties and phylogenetic inference indicated that WDS2C27 could be assigned to a novel species within a novel genus, for which the name gen. nov., sp. nov. is proposed. Strain WDS2C27 (=MCCC 1H00133=KCTC 52044) is the type strain.
一株革兰氏阴性、非运动、细杆或短丝形状、紫晶色素的细菌，被命名为 WDS2C27 菌株，从中国威海文登的一个海洋太阳能盐场中分离得到（37°31'5″N，122°1'47″E）。WDS2C27 的生长温度为 20-42°C（最适 37°C），pH 值为 6.5-8.5（最佳 pH 值为 7.0-8.0）。在含有 6%（w/v）NaCl 的改良海洋肉汤中生长最佳。WDS2C27 的主要极性脂为磷脂酰乙醇胺、两种未鉴定的氨基脂和一种未鉴定的脂。WDS2C27 的主要呼吸醌为 MK-6。优势脂肪酸为 iso-C 和 anteisoC。DNA G+C 含量为 35.0 mol%。16S rRNA 基因的核苷酸序列表明，最接近的菌株是 X15M-8（1452 bp 序列中有 92.0%相似）。WDS2C27 与属内模式种的平均氨基酸同一性为 60.7%，保守蛋白百分比为 55.6%，平均核苷酸同一性为 75.0%，数字 DNA-DNA 杂交同一性为 13.1%，属模式种为 ，ATCC 700755。表型和基因型特征以及系统发育推断表明，WDS2C27 可归入一个新属的一个新种，因此建议使用新属名和新种名 ，sp. nov.。WDS2C27 菌株（=MCCC 1H00133=KCTC 52044）是模式菌株。
