State Key Laboratory of Microbial Technology, Institute of Microbiology Technology, Shandong University, Qingdao 266237, PR China.
Int J Syst Evol Microbiol. 2020 Dec;70(12):6284-6293. doi: 10.1099/ijsem.0.004527.
A Gram-stain-negative, strictly aerobic, non-motile, orange-coloured bacterium, designated YR1-1, was isolated from a soil sample collected from the Yellow River Delta wetlands (PR China). Growth was observed at a salinity of 1.0-15.0 % NaCl, 4-45 °C and pH 6.0-9.0. The results of phylogenetic analysis based on the 16S rRNA gene sequences indicated that YR1-1 represented a member of the genus , with the highest sequence similarity to YIM-C238 (97.9 %), followed by (97.1 %) and (96.4 %). The average nucleotide identity and digital DNA-DNA hybridization values between YR1-1 and other closely related type strains of species of the genus were 68.7-86.3% and 17.8-30.9 %. The genome of the strain was 2 899 374 bp in length with 39.8 % DNA G+C content. The predominant fatty acids (>10 %) were iso-C and anteiso-C. The major respiratory quinone was menaquinone-6 (MK-6) and the major polar lipids were phosphatidylethanolamine, phospholipid, diphosphatidylglycerol, two unidentified aminolipids and four unidentified lipids. The combined genotypic and phenotypic data indicate that YR1-1 represents a novel species within the genus , for which the name sp. nov., is proposed. The type strain is YR1-1 (=KCTC 72794=CGMCC 1.17458).
一株革兰氏阴性、严格需氧、非运动、橙色的细菌，被命名为 YR1-1，从中国黄河三角洲湿地的土壤样本中分离得到。在盐度为 1.0-15.0%NaCl、4-45°C 和 pH 值 6.0-9.0 的条件下观察到生长。基于 16S rRNA 基因序列的系统发育分析结果表明，YR1-1 是属的一个成员，与 YIM-C238（97.9%）的序列相似度最高，其次是 （97.1%）和 （96.4%）。YR1-1 与其他密切相关的属种的平均核苷酸同一性和数字 DNA-DNA 杂交值在 68.7-86.3%和 17.8-30.9%之间。该菌株的基因组长度为 2899374bp，GC 含量为 39.8%。优势脂肪酸（>10%）为 iso-C 和 anteiso-C。主要呼吸醌为menaquinone-6 (MK-6)，主要极性脂为磷脂酰乙醇胺、磷脂、双磷脂酰甘油、两种未鉴定的胺脂和四种未鉴定的脂。综合表型和基因型数据表明，YR1-1 代表属内的一个新种，建议将其命名为 sp. nov.。模式菌株为 YR1-1（=KCTC 72794=CGMCC 1.17458）。
