Khonkhaeng Benjamad, Cherdthong Anusorn
Tropical Feed Resources Research and Development Center (TROFREC), Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand.
J Fungi (Basel). 2020 May 21;6(2):69. doi: 10.3390/jof6020069.
It was hypothesized that white-rot fungus fermented with rice straw and purple field corn improves nutrient utilization via enhanced digestibility and lowers methane (CH) production due to the effects of the lovastatin compound. The aim of the current experiment was to investigate the effect of inoculation of two fungi belonging to white-rot fungus type on feed value and ruminal fermentation characteristic. The experiment was carried out according to a completely randomized 3 × 3 factorial design: three roughage sources (rice straw, purple corn stover, and purple corn field cob) for three inoculation methods (untreated, treated, and treated). The two fungi increased concentration of lovastatin when compared to the untreated, and had higher lovastatin production potential than ( < 0.05). The yield of lovastatin was obtained from rice straw fermentation with . The monomeric anthocyanin content (MAC) in untreated purple field corn cobs was higher than in the fermentation groups. Ruminal fermentation gas production from soluble fractions ranged from -2.47 to 1.14 and differed among the treatments ( < 0.01). In comparison to all treatments, the gas production rate for the insoluble fraction was significantly highest ( < 0.01) in treatment alone, in which purple field corn stover was fermented with and . There was significant interaction in in vitro dry matter digestibility at 12 h of incubation. Purple field corn cob had a higher significant effect on in vitro DM digestibility at 12 and 24 h after incubation when compared to that of other groups. Moreover, current research has found that roughage fermented with and increased in vitro DM digestibility at 24 h after incubation. Fermenting roughage with fungi did not affect rumen pH, which ranged from 6.60 to 6.91 ( > 0.05), while resulted in increased levels of ruminal ammonia-nitrogen concentrations. Propionic acid increased in all roughages fermented with or after 8 h of ruminal fermentation testing. The two fungi fermented as substrate treatments had significantly lower ( < 0.05) CH production. Based on the improved rumen DM digestibility and reduced CH production, and could be utilized for enhancing feeding efficiency of roughage.
据推测，用稻草和紫玉米发酵的白腐真菌可通过提高消化率来改善养分利用，并由于洛伐他汀化合物的作用降低甲烷（CH）的产生。本实验的目的是研究接种两种白腐真菌对饲料价值和瘤胃发酵特性的影响。实验按照完全随机的3×3析因设计进行：三种粗饲料来源（稻草、紫玉米秸秆和紫玉米田间果穗）对应三种接种方法（未处理、处理和处理）。与未处理组相比，这两种真菌提高了洛伐他汀的浓度，且比具有更高的洛伐他汀生产潜力（P<0.05）。洛伐他汀产量来自用发酵的稻草。未处理的紫玉米果穗中的单体花青素含量（MAC）高于发酵组。瘤胃发酵气体中可溶部分的产生量在-2.47至1.14之间，且各处理间存在差异（P<0.01）。与所有处理相比，单独处理（紫玉米秸秆用和发酵）中不溶部分的产气率显著最高（P<0.01）。培养12小时时体外干物质消化率存在显著交互作用。与其他组相比，紫玉米果穗在培养12小时和24小时后对体外干物质消化率有更高的显著影响。此外，当前研究发现，用和发酵的粗饲料在培养24小时后体外干物质消化率提高。用真菌发酵粗饲料不影响瘤胃pH值，其范围在6.60至6.91之间（P>0.05），而导致瘤胃氨氮浓度升高。瘤胃发酵测试8小时后，所有用或发酵的粗饲料中丙酸增加。作为底物处理发酵的这两种真菌产生的CH显著更低（P<0.05）。基于改善的瘤胃干物质消化率和降低的CH产生，和可用于提高粗饲料的饲喂效率。
