Allimuthu Elangovan, Dalal Monika, Kumar Krishna Gopinathan, Sellathdurai Devika, Kumar Ranjeet Ranjan, Sathee Lekshmy, Chinnusamy Viswanathan
Division of Plant Physiology, ICAR-Indian Agricultural Research Institute, New Delhi-110012, India.
ICAR-National Institute for Plant Biotechnology, New Delhi-110012, India.
Plants (Basel). 2020 May 23;9(5):664. doi: 10.3390/plants9050664.
Tyrosine phosphorylation constitutes up to 5% of the total phophoproteome. However, only limited studies are available on protein tyrosine kinases (PTKs) that catalyze protein tyrosine phosphorylation in plants. In this study, domain analysis of the 27 annotated genes in rice genome led to the identification of 18 PTKs with tyrosine kinase domain. The kinase domain of rice PTKs shared high homology with that of dual specificity kinase BRASSINOSTEROID- INSENSITIVE 1 (BRI1) of . In phylogenetic analysis, rice PTKs clustered with receptor-like cytoplasmic kinases-VII (RLCKs-VII) of . mRNAseq analysis using Genevestigator revealed that rice except and express at moderate to high level in most tissues. expression was highly abundant in panicle at flowering stage. mRNAseq data analysis led to the identification of drought, heat, salt, and submergence stress regulated genes in rice. was upregulated under all stresses. qRT-PCR analysis also showed that all except were significantly upregulated in root under osmotic stress. Tissue specificity and abiotic stress mediated differential regulation of suggest their potential role in development and stress response of rice. The candidate dual specificity identified in this study paves way for molecular analysis of tyrosine phosphorylation in rice.
酪氨酸磷酸化占总磷酸化蛋白质组的比例高达5%。然而，关于催化植物中蛋白质酪氨酸磷酸化的蛋白质酪氨酸激酶（PTK）的研究却很有限。在本研究中，对水稻基因组中27个注释基因进行结构域分析，鉴定出18个具有酪氨酸激酶结构域的PTK。水稻PTK的激酶结构域与拟南芥双特异性激酶油菜素内酯不敏感1（BRI1）的激酶结构域具有高度同源性。在系统发育分析中，水稻PTK与拟南芥受体样细胞质激酶-VII（RLCKs-VII）聚类。使用Genevestigator进行的mRNAseq分析表明，除LOC_Os03g59230和LOC_Os06g42100外，水稻PTK在大多数组织中表达水平为中度至高。LOC_Os03g59230在开花期的穗中表达量非常丰富。mRNAseq数据分析鉴定出水稻中受干旱、高温、盐和淹水胁迫调控的PTK基因。LOC_Os03g59230在所有胁迫下均上调。qRT-PCR分析还表明，除LOC_Os06g42100外，所有PTK在渗透胁迫下的根中均显著上调。PTK的组织特异性和非生物胁迫介导的差异调控表明它们在水稻发育和胁迫响应中具有潜在作用。本研究中鉴定出的候选双特异性PTK为水稻酪氨酸磷酸化的分子分析铺平了道路。
