Li Ronghua, Zhang Weijia, Lu Junkai, Zhang Zhouyi, Mu Changkao, Song Weiwei, Migaud Herve, Wang Chunlin, Bekaert Michaël
Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo, China.
Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo University, Ningbo, China.
Front Genet. 2020 May 8;11:440. doi: 10.3389/fgene.2020.00440. eCollection 2020.
The hard-shelled mussel () is an economically important shellfish that has been cultivated for the last decade. Due to over-exploitation, most mussel stocks have dramatically declined. Efforts to study this species' natural distribution, genetics, breeding, and cultivation have been hindered by the lack of a high-quality reference genome. To address this, we produced a hybrid high-quality reference genome of using a long-read platform to assemble the genome and short-read, high-quality technology to accurately correct for sequence errors. The genome was assembled into 10,484 scaffolds, a total length of 1.90 Gb, and a scaffold N50 of 898 kb. annotation of the genome assembly identified a total of 42,684 genes. This accurate reference genome of provides an essential resource with the advantage of enabling the genome-scale selective breeding of . More importantly, it will also help in deciphering the speciation and local adaptation of the species.
硬壳贻贝()是一种具有重要经济价值的贝类,在过去十年中一直被养殖。由于过度开发,大多数贻贝种群数量急剧下降。由于缺乏高质量的参考基因组,对该物种自然分布、遗传学、育种和养殖的研究工作受到了阻碍。为了解决这一问题,我们利用长读长平台组装基因组,并结合短读长、高质量技术对序列错误进行精确校正,从而产生了一个硬壳贻贝的高质量杂交参考基因组。该基因组被组装成10484个支架,总长度为19.0Gb,支架N50为898kb。对硬壳贻贝基因组组装的注释共鉴定出42684个基因。这个精确的硬壳贻贝参考基因组提供了一个重要资源,具有实现硬壳贻贝基因组规模选择育种的优势。更重要的是,它还将有助于解读硬壳贻贝物种的物种形成和局部适应性。