Fallah Fatemeh, Borhan Rebwar Shams, Hashemi Ali
Pediatric Infectious Research Center, Mofid Children Hospital, Shahid Beheshti University of Medical Sciences Tehran, Iran.
Int J Burns Trauma. 2013 Apr 18;3(2):122-4. Print 2013.
Acquired Metallo-β-Lactamases (MBLs) are emerging resistance determinants in Pseudomonas aeruginosa and other gram-negative bacteria.Using Combination Disk Diffusion test, it was found that among 83 imipenem non-susceptible P. aeruginosa strains, 48 (57.9%) were MBL producers. PCR and Sequencing methods proved that these isolates were positive for blaIMP-1 genes, whereas none were positive for bla(VIM) genes. The mortality rate due to MBL-producing Pseudomonas infection was 4 (8.3%) among the hospitalized patients. Therefore, identification of drug resistance patterns in P. aeruginosa and detection of MBLs producing isolates are of great importance in the prevention and control of infections.
获得性金属β-内酰胺酶（MBLs）是铜绿假单胞菌和其他革兰氏阴性菌中新兴的耐药决定因素。通过联合纸片扩散试验发现，在83株对亚胺培南不敏感的铜绿假单胞菌菌株中，有48株（57.9%）是MBL产生菌。PCR和测序方法证明这些分离株blaIMP-1基因呈阳性，而bla(VIM)基因均为阴性。在住院患者中，由产MBL的铜绿假单胞菌感染导致的死亡率为4例（8.3%）。因此，鉴定铜绿假单胞菌的耐药模式并检测产MBL的分离株对于感染的预防和控制至关重要。
