Center of Reproductive Medicine, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, 510623, Guangzhou, Guangdong, P.R. China.
Department of Gynecological Oncology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, 510623, Guangzhou, Guangdong, P.R. China.
Reprod Biol Endocrinol. 2020 May 27;18(1):55. doi: 10.1186/s12958-020-00615-x.
The effects of cigarette smoking on male semen quality are controversial, and the molecular mechanisms underlying how cigarette smoking affects semen quality are not clear yet.
In this study, semen samples from 70 heavy smokers and 75 non-smokers receiving infertility treatment were included. Basic semen parameters in non-smokers and heavy smokers were evaluated. Levels of glutathione (GSH), lipid reactive oxygen species (ROS), iron and GSH-dependent peroxidase 4 (GPX4) protein level were observed in human seminal plasma and in GC-2Spd cells exposed to cigarette smoke condensate (CSC).
Heavy smokers had significantly higher abnormalities (sperm viability and sperm progressive motility) than non-smoking counterparts. Comparing non-smokers group, GSH level was reduced in the group of heavy smokers (P < 0.05). However, the level of lipid ROS and iron were significantly increased (P < 0.05). Besides, GSH level was reduced following treatment with CSC for 24 h, while lipid ROS and iron levels were increased (P < 0.05). However, the levels were reduced after being co-cultured with Ferrostatin-1 (Fer-1) (P < 0.05). The level of GPX4 protein was reduced after being treated with CSC in 24 h, and increased after being co-cultured with Fer-1(P < 0.05).
Cigarette smoking is associated with high level of ferroptosis in seminal plasma and affect semen quality.
吸烟对男性精液质量的影响存在争议，吸烟影响精液质量的分子机制尚不清楚。
本研究纳入了 70 名重度吸烟者和 75 名接受不育治疗的非吸烟者的精液样本。评估了非吸烟者和重度吸烟者的基本精液参数。观察了人精液和暴露于香烟烟雾冷凝物（CSC）的 GC-2Spd 细胞中的谷胱甘肽（GSH）、脂质活性氧（ROS）、铁和 GSH 依赖性过氧化物酶 4（GPX4）蛋白水平。
重度吸烟者的异常（精子活力和精子前向运动）明显高于非吸烟者。与非吸烟者组相比，重度吸烟者组的 GSH 水平降低（P<0.05）。然而，脂质 ROS 和铁的水平显著增加（P<0.05）。此外，CSC 处理 24 小时后 GSH 水平降低，脂质 ROS 和铁水平升高（P<0.05）。然而，与 Ferrostatin-1（Fer-1）共培养后水平降低（P<0.05）。CSC 处理 24 小时后 GPX4 蛋白水平降低，与 Fer-1 共培养后水平升高（P<0.05）。
吸烟与精液中高水平的铁死亡有关，并影响精液质量。
