Barros Silvana, Ribeiro Ana Paula D, Offenbacher Steven, Loewy Zvi G
Department of Periodontology, School of Dentistry, University of North Carolina, Chapel Hill, NC 27599, USA.
Department of Restorative Dental Sciences, College of Dentistry, University of Florida, Gainesville, FL 32610, USA.
Microorganisms. 2020 May 26;8(6):804. doi: 10.3390/microorganisms8060804.
Oral mucositis, inflammation, and ulceration that occur in the oral cavity can manifest in significant pain. A formulation was designed to investigate the potential of vitamin E to ameliorate inflammation resulting from in cell-based systems. Human gingival fibroblasts and THP1 cells were stimulated with heat killed and LPS (agonists). Unstimulated cells were included as controls. Cells were also simultaneously treated with a novel denture adhesive formulation that contains vitamin E (antagonist). The experimental conditions included cells exposed to the experimental formulation or the vehicle for 2 h for mRNA extraction and analysis, and cells left for 24 h under those experimental conditions for analysis of protein expression by ELISA. ssAffymetrix expression microarray pathway analyses demonstrated that the tested formulation exhibited a statistically significant ( < 0.05) inhibition of the following key inflammatory pathways: TLR 6, IL-1 signaling (IRAK, A20), NF-kappaB, IL-6 signaling (gp130, JK2 and GRB2), TNF signaling (TNF receptor) and Arachidonic acid metabolism (PLA2). Quantitative PCR array analysis confirmed the downregulation of key inflammatory genes when cells under adhesive treatment were challenged with heat killed . PGE2 secretion was inhibited by the tested formulation only on THP1 cells after 24 h stimulation with . These results suggest that the active formulation containing vitamin E acetate can modulate inflammatory responses, through anti-inflammatory actions as indicated by in vitro experimental conditions.
口腔黏膜炎、炎症以及口腔内出现的溃疡会引发剧痛。设计了一种配方来研究维生素E在细胞系统中减轻炎症的潜力。用人牙龈成纤维细胞和THP1细胞，用热灭活的[具体物质]和脂多糖（激动剂）进行刺激。未刺激的细胞作为对照。细胞同时用一种含有维生素E（拮抗剂）的新型义齿黏附剂配方进行处理。实验条件包括将细胞暴露于实验配方或赋形剂中2小时以提取和分析mRNA，以及在这些实验条件下将细胞放置24小时以通过酶联免疫吸附测定法分析蛋白质表达。单通道基因芯片表达微阵列通路分析表明，所测试的配方对以下关键炎症通路表现出统计学上显著的（<0.05）抑制作用：Toll样受体6、白细胞介素-1信号通路（白细胞介素-1受体相关激酶、A20）、核因子κB、白细胞介素-6信号通路（糖蛋白130、JAK2和生长因子受体结合蛋白2）、肿瘤坏死因子信号通路（肿瘤坏死因子受体）和花生四烯酸代谢（磷脂酶A2）。定量PCR阵列分析证实，当用热灭活的[具体物质]刺激黏附处理的细胞时，关键炎症基因的表达下调。仅在用[具体物质]刺激24小时后，所测试的配方在THP1细胞上抑制了前列腺素E2的分泌。这些结果表明，含醋酸维生素E的活性配方可通过体外实验条件所示的抗炎作用来调节炎症反应。
