纳米颗粒的介导生物合成与生物强化
mediated biosynthesis of nanoparticles and biofortification.
作者信息
Rana Shivnam, Kapoor Shammi, Sharma Shivani, Kalia Anu
机构信息
Department of Microbiology, College of Basic Sciences and Humanities, Punjab Agricultural University, Ludhiana, Punjab 141004 India.
Electron Microscopy and Nanoscience Laboratory, Department of Soil Science, College of Agriculture, Punjab Agricultural University, Ludhiana, Punjab 141004 India.
出版信息
Food Sci Biotechnol. 2023 Apr 26;32(14):2079-2092. doi: 10.1007/s10068-023-01307-z. eCollection 2023 Dec.
UNLABELLED
The mycosynthesis of biogenic NPs using nanotechnology technique is an ecofriendly and economical approach. The extracellular mycelial extract of the fungi were used to biosynthesized Zn, Cu and Fe NPs using zinc sulphate, zinc chloride, copper sulphate, copper chloride ferrous sulphate and ferric chloride, precursor salts at 1.0 mM concentration. The color of reaction mixture was changed from (transparent to white, blue to green and yellow to brown) for Zn, Cu and Fe NPs during incubation period of 96 h at 25 ± 2 °C, indicating synthesis of NPs. Spectroscopy and microscopy techniques were used for the characterization of newly synthesized biogenic NPs. Whereas, the ICP-MS analysis revealed that copper chloride precursor salts produced high concentration of Cu biogenic NPs, followed by zinc chloride derived Zn NPs. The fortification with the biogenic NPs of mycelium exhibited high accumulation of the trace elements as compared to non-fortified mycelium.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s10068-023-01307-z.
未标注
利用纳米技术进行生物源纳米颗粒的真菌合成是一种环保且经济的方法。使用硫酸锌、氯化锌、硫酸铜、氯化铜、硫酸亚铁和氯化铁等浓度为1.0 mM的前体盐，真菌的细胞外菌丝提取物被用于生物合成锌、铜和铁纳米颗粒。在25±2°C下孵育96小时期间，锌、铜和铁纳米颗粒的反应混合物颜色从（透明变为白色、蓝色变为绿色、黄色变为棕色），表明纳米颗粒已合成。光谱学和显微镜技术用于对新合成的生物源纳米颗粒进行表征。而电感耦合等离子体质谱分析表明，氯化铜前体盐产生的铜生物源纳米颗粒浓度较高，其次是氯化锌衍生的锌纳米颗粒。与未强化的菌丝体相比，用生物源纳米颗粒强化的菌丝体表现出较高的微量元素积累。
补充信息
在线版本包含可在10.1007/s10068-023-01307-z获取的补充材料。
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