Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand; Center of Excellence in Microbial Diversity and Suitable Utilization, Chiang Mai University, Chiang Mai, 50200, Thailand.
Center of Excellence in Microbial Diversity and Suitable Utilization, Chiang Mai University, Chiang Mai, 50200, Thailand.
Int J Med Mushrooms. 2020;22(3):221-233. doi: 10.1615/IntJMedMushrooms.2020034092.
Bioactive polysaccharides from three wild edible mushrooms (Rugibolutus extremiorientalis, Russula emetica, and Phleobopus portentosus) extracted by conventional refluxing extraction (RFE) and ultrasound-assisted extraction (UAE) were investigated. The result indicated that the PS of R. extremiorientalis extracted by RFE exhibited the greatest antioxidant activities on ABTS cation free radical scavenging (153.0 mg TEAC/g of the extract), DPPH radical scavenging (9.91 mg TEAC/g of the extract), and also reducing power capacity (10.2 mg TEAC/g of the extract). Whereas, those of R. emetica extracted by UAE have the highest antidiabetic and antihypertensive activities (56.3% and 70.5% inhibition, respectively). All crude PS extracts were composed mainly of carbohydrate and protein plus a small proportion of phenolic compounds of which glucose was the prevalent monosaccharide of all polysaccharides. PS of both R. extremiorientalis and R. emetica contained of β-glycosidic conformation, while those of Ph. portentosus contained both α- and β-glycosidic conformations.
从三种野生食用蘑菇（硬皮地星、红绒盖牛肝菌和粗皮側耳）中用常规回流提取（RFE）和超声辅助提取（UAE）提取的生物活性多糖进行了研究。结果表明，用 RFE 提取的 R. extremiorientalis 的 PS 对 ABTS 阳离子自由基清除（提取物中 153.0mgTEAC/g）、DPPH 自由基清除（提取物中 9.91mgTEAC/g）和还原能力（提取物中 10.2mgTEAC/g）具有最大的抗氧化活性。然而，用 UAE 提取的 R. emetica 的 PS 具有最高的抗糖尿病和降血压活性（分别抑制 56.3%和 70.5%）。所有粗 PS 提取物主要由碳水化合物和蛋白质组成，加上少量酚类化合物，其中葡萄糖是所有多糖的常见单糖。R. extremiorientalis 和 R. emetica 的 PS 均含有β-糖苷键构象，而 Ph. portentosus 的 PS 则含有α-和β-糖苷键构象。
