Department of Veterinary Medicine, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Tokyo, Japan.
J Biomater Appl. 2022 Jul;37(1):3-11. doi: 10.1177/0885328220928660. Epub 2020 Jun 2.
Medical sheets are useful in surgically repair vascular disease. To avoid long-term side effects, they are to be replaced with regenerated tissue after implantation. Silk fibroin is a fibrous protein secreted by silkworm. The advantage of silk fibroin is its biocompatibility and has been used as regenerative artificial materials. The problem of its biodegradability is that the effect is time consuming. In this study, SVVYGLR peptide was used to expect promoting cell migration and accelerating the biodegradation of silk fibroin. Silk fibroin and polyurethane-based medical sheets with or without SVVYGLR peptide were implanted in rat abdominal aorta (silk fibroin/polyurethane/SVVYGLR peptide versus silk fibroin/polyurethane). The result of histological evaluation indicated that the new cell layer created under both sheets was composed of endothelial cells, smooth muscle, and fibroin in both sheets and similar to a native vessel. Both sheets did not show any excessive inflammation or calcification, and moderate biodegradability was observed. The decrease of silk fibroin indicated the biodegradability of all sheets. Silk fibroin/polyurethane/SVVYGLR peptide had many small vessels in the regenerated tissue than silk fibroin/polyurethane. This appearance indicated that SVVYGLR peptide promoted the angiogenesis in the regenerative tissue. This study suggested that SVVYGLR peptide could give the angiogenic-promoting activity to silk fibroin-based vascular repairing sheet.
医用补片在血管疾病的外科修复中很有用。为了避免长期的副作用，在植入后，它们需要被再生组织所取代。丝素蛋白是一种由蚕分泌的纤维状蛋白质。丝素蛋白的优点是其生物相容性，并已被用作再生人工材料。其生物降解性的问题是效果耗时。在这项研究中，使用 SVVYGLR 肽来促进细胞迁移并加速丝素蛋白的生物降解。将含有或不含有 SVVYGLR 肽的丝素蛋白和聚氨酯基医用补片植入大鼠腹主动脉（丝素蛋白/聚氨酯/SVVYGLR 肽与丝素蛋白/聚氨酯）。组织学评估的结果表明，两种补片下形成的新细胞层均由内皮细胞、平滑肌和纤维蛋白组成，类似于天然血管。两种补片均未显示出任何过度的炎症或钙化，且观察到适度的生物降解性。丝素蛋白的减少表明所有补片均具有生物降解性。丝素蛋白/聚氨酯/SVVYGLR 肽在再生组织中的小血管比丝素蛋白/聚氨酯多。这种外观表明 SVVYGLR 肽促进了再生组织中的血管生成。本研究表明，SVVYGLR 肽可以为丝素基血管修复补片提供促进血管生成的活性。
