Sheet Partha S, Koley Dipankar
Department of Chemistry, Oregon State University, Corvallis, OR 97331, USA.
ACS Appl Bio Mater. 2019 Dec 16;2(12):5941-5948. doi: 10.1021/acsabm.9b00866. Epub 2019 Nov 15.
A glucose-modified dendritic hydrogel is used as a bioink for bacterial encapsulation. This biocompatible hydrogel is a potentially suitable alternative to conventional alginate hydrogel for bacterial encapsulation, as it readily forms gel in the presence of Na or K ions without any additional stimuli such as pH, temperature, sonication, or the presence of divalent metal ions. We created a bacterial microhabitat by adding the gelator to phosphate-buffered saline containing live bacteria at physiological pH and using an additive three-dimensional (3D) printing technique. The bacteria remained viable and metabolically active within the 3D printed bacterial microhabitat, as shown with confocal laser scanning microscopy (CLSM) and scanning electrochemical microscopy (SECM).
一种葡萄糖修饰的树枝状水凝胶被用作包封细菌的生物墨水。这种生物相容性水凝胶是用于细菌包封的传统藻酸盐水凝胶的潜在合适替代品，因为它在存在Na或K离子的情况下无需任何额外刺激（如pH、温度、超声处理或二价金属离子的存在）就能轻松形成凝胶。我们通过将凝胶剂添加到生理pH下含有活细菌的磷酸盐缓冲盐水中，并使用添加剂三维（3D）打印技术创建了一个细菌微环境。如共聚焦激光扫描显微镜（CLSM）和扫描电化学显微镜（SECM）所示，细菌在3D打印的细菌微环境中保持存活且代谢活跃。
