Yin Panjing, Su Weiwei, Li Ting, Wang Ling, Pan Jianying, Wu Xiaoqi, Shao Yan, Chen Huabin, Lin Lin, Yang Yang, Cheng Xiulin, Li Yanbing, Wu Yaobin, Zeng Chun, Huang Wenhua
Department of Joint Surgery, The Third Affiliated Hospital, Southern Medical University, Guangzhou 510630, P.R.China.
Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, Guangdong Provincial Key Laboratory of Digital Medicine and Biomechanics, National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.
iScience. 2023 Jul 11;26(8):107349. doi: 10.1016/j.isci.2023.107349. eCollection 2023 Aug 18.
Articular cartilage tissue engineering is being considered an alternative treatment strategy for promoting cartilage damage repair. Herein, we proposed a modular hydrogel-based bioink containing microsphere-embedded chondrocytes for 3D printing multiscale scaffolds integrating the micro and macro environment of the native articular cartilage. Gelatin methacryloyl (GelMA)/alginate microsphere was prepared by a microfluidic approach, and the chondrocytes embedded in the microspheres remained viable after being frozen and resuscitated. The modular hydrogel bioink could be printed via the gel-in-gel 3D bioprinting strategy for fabricating the multiscale hydrogel-based scaffolds. Meanwhile, the cells cultured in the scaffolds showed good proliferation and differentiation. Furthermore, we also found that the composite hydrogel was biocompatible . These results indicated that the modular hydrogel-based bioinks containing microsphere-embedded chondrocytes for 3D printing multiscale scaffolds could provide a 3D multiscale environment for enhancing cartilage repairing, which would be encouraging considering the numerous alternative applications in articular cartilage tissue engineering.
关节软骨组织工程被认为是促进软骨损伤修复的一种替代治疗策略。在此，我们提出了一种基于模块化水凝胶的生物墨水，其包含微球包埋的软骨细胞，用于3D打印整合天然关节软骨微观和宏观环境的多尺度支架。通过微流控方法制备了甲基丙烯酰化明胶（GelMA）/藻酸盐微球，包埋在微球中的软骨细胞在冷冻复苏后仍保持活力。模块化水凝胶生物墨水可通过凝胶包凝胶3D生物打印策略进行打印，以制造基于多尺度水凝胶的支架。同时，在支架中培养的细胞表现出良好的增殖和分化。此外，我们还发现复合水凝胶具有生物相容性。这些结果表明，用于3D打印多尺度支架的含微球包埋软骨细胞的模块化水凝胶生物墨水可为增强软骨修复提供三维多尺度环境，考虑到其在关节软骨组织工程中的众多替代应用，这将是令人鼓舞的。
