Dini Irene, Graziani Giulia, Fedele Francesca Luisa, Sicari Andrea, Vinale Francesco, Castaldo Luigi, Ritieni Alberto
Department of Pharmacy, University of Naples Federico II, Via Domenico Montesano 49, 80141 Naples, Italy.
Linfa scarl, Via Zona Industriale Porto San Salvo, 89900 Vibo Valentia, Italy.
Antioxidants (Basel). 2020 Jun 1;9(6):466. doi: 10.3390/antiox9060466.
In the Rural Development Plan (2014-2020), the European Commission encouraged the conversion and supported the maintenance of organic farming. Organic olive oil (bioEVOO) production involves the use of environmentally sustainable fertilizers and the recycling of olive pomace (Pom) and olive vegetation waters (VW) to reduce the environmental impact of these wastes. An ecofriendly way to recycle olive wastes is to reuse them to extract bioactive compounds. In this study, the total phenolic compounds content, their profile and dosage, the antioxidant action in oil, pomace, and vegetation water was evaluated when the M10 was used as a biostimulant in agriculture. Two spectrophotometric tests (2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic) acid (ABTS)) evaluated the antioxidant potential of samples, a spectrophotometric method estimated total phenolic content, and an Ultra-High-Performance Liquid Chromatography (UHPLC)-Orbitrap method evaluated the phenolics profile. Our results showed that the biostimulation improved the antioxidant potential and the total concentration of phenolics in the bioEVOO and bio-pomace (bioPom) samples and mainly enhanced, among all classes of phenolic compounds, the production of the flavonoids and the secoiridoids. Moreover, they demonstrated the action in the mevalonate pathway to produce phenols for the first time. The decisive action of the on the production of phenolic compounds increases the economic value of the waste materials as a source of bioactive compounds useful for the pharmaceutical, cosmetic, and food industries.
在《农村发展计划（2014 - 2020）》中，欧盟委员会鼓励有机农业的转型并支持其维持。有机橄榄油（bioEVOO）生产涉及使用环境可持续肥料以及橄榄果渣（Pom）和橄榄树种植废水（VW）的循环利用，以减少这些废弃物对环境的影响。一种环保的橄榄废弃物循环利用方式是将其再利用以提取生物活性化合物。在本研究中，当将M10用作农业生物刺激剂时，评估了其在橄榄油、果渣和种植废水中的总酚类化合物含量、其谱图及剂量以及抗氧化作用。两种分光光度法测试（2,2 - 二苯基 - 1 - 苦基肼自由基（DPPH）和2,2'- 偶氮二（3 - 乙基苯并噻唑啉 - 6 - 磺酸）酸（ABTS））评估了样品的抗氧化潜力，一种分光光度法估算总酚含量，超高效液相色谱（UHPLC）- 轨道阱法评估酚类化合物谱图。我们的结果表明，生物刺激提高了bioEVOO和生物果渣（bioPom）样品中的抗氧化潜力和酚类化合物的总浓度，并且在所有酚类化合物类别中，主要增强了黄酮类化合物和裂环烯醚萜类化合物的生成。此外，它们首次证明了在甲羟戊酸途径中对酚类化合物生成的作用。M10对酚类化合物生成的决定性作用提高了作为制药、化妆品和食品工业有用生物活性化合物来源的废料的经济价值。
