Alsubki Roua, Tabassum Hajera, Alfawaz Hala, Alaqil Rasha, Aljaser Feda, Ansar Sabah, Al Jurayyan Abdullah
Department of Clinical Laboratory Sciences, Chair of Medical and Molecular Genetics Research, College of Applied Medical Sciences, King Saud University, Riyadh, Kingdom of Saudi Arabia.
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Kingdom of Saudi Arabia.
Intractable Rare Dis Res. 2020 May;9(2):89-94. doi: 10.5582/irdr.2020.03012.
Antinuclear antibodies (ANA) and extractable nuclear antigens (ENA) are instrumental biomarkers crucial in the detection of autoimmune disorders (AID) such as systemic lupus erythematosus (SLE), Sjogren syndrome, . In the present study, an assessment of the most frequent ANA patterns associated with most detectable ENA that could be used as efficient prognostic markers in the diagnosis of autoimmune diseases was conducted. Data was retrospectively analyzed from AID patients, retrieved from the medical records of King Fahad Medical City, Riyadh, KSA, from January 2016 to October 2018 who underwent ANA immunofluorescence of HEp-2 cells and their ENA detection was studied. Of the 453 total patients, 39/55 AID males (71%) and 332/398 AID females (83.4%) exhibited ANA positivity. The most common pattern was speckled S-ANA (32.4%) in females and homogenous H-ANA pattern (25.4%) in males. The histones were found at higher frequency in different ANA patterns. anti-Sjogren syndrome related antigen A (SSA), anti-ribonucleoprotein antibody (RNP-Sm), and histones were observed to be associated with homogenous and speckled nuclear patterns. Frequencies of ENA in all ANA patterns were found significant at < 0.05 in males and < 0.001 in females. Spearman's rank correlation of ENA within and among the ANA patterns was non-significant. SSA was significantly correlated with RNP-Sm and Sm at < 0.05 and < 0.01, respectively. The extractable nuclear antigens SSA, RNP-Sm, and histones were found associated with the S-ANA and H-ANA patterns. These correlations are of relevance for the accurate diagnosis of autoimmune diseases.
抗核抗体（ANA）和可提取核抗原（ENA）是检测自身免疫性疾病（AID）如系统性红斑狼疮（SLE）、干燥综合征等的重要生物标志物。在本研究中，对与最易检测到的ENA相关的最常见ANA模式进行了评估，这些模式可作为自身免疫性疾病诊断中的有效预后标志物。对2016年1月至2018年10月在沙特阿拉伯利雅得法赫德国王医疗城接受HEp-2细胞ANA免疫荧光检测及ENA检测的AID患者的病历数据进行回顾性分析。在453例患者中，55例AID男性中有39例（71%）和398例AID女性中有332例（83.4%）ANA呈阳性。女性中最常见的模式是斑点状S-ANA（32.4%），男性中是均质型H-ANA模式（25.4%）。在不同的ANA模式中发现组蛋白的频率较高。抗干燥综合征相关抗原A（SSA）、抗核糖核蛋白抗体（RNP-Sm）和组蛋白与均质型和斑点状核模式相关。在男性中，所有ANA模式下ENA的频率在<0.05时具有显著性，在女性中在<0.001时具有显著性。ANA模式内和模式间ENA的Spearman等级相关性不显著。SSA分别与RNP-Sm和Sm在<0.05和<0.01时显著相关。发现可提取核抗原SSA、RNP-Sm和组蛋白与S-ANA和H-ANA模式相关。这些相关性对于自身免疫性疾病的准确诊断具有重要意义。
