Department of Pharmacy, Qilu Hospital of Shandong University, Jinan, China.
Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China.
Phytochem Anal. 2020 Nov;31(6):835-845. doi: 10.1002/pca.2949. Epub 2020 Jun 3.
Angiogenesis is closely related to a variety of diseases, and therapies based on angiogenesis are intensely investigated. Studies have shown that the use of Gastrodiae Rhizoma (GR, Gastrodia elata) can benefit the treatment of ischemic cardiovascular diseases and atherosclerosis by stimulating angiogenesis.
This study tested the angiogenesis effects of a group of chemical markers isolated from GR.
Zebrafish model was used to evaluate angiogenesis by setting four groups: blank control group, model group, positive control group and treatment group (0.1, 1, and 100 μg/mL RGP). The Gray correlation analysis (GCA) was implemented to calculate the correlation coefficients of each compound between the peak area in liquid chromatography time-of-flight mass spectrometry (LC-TOF-MS) and the bioactivity, the top ten components with the correlation degree > 0.9 were listed.
The optimum final concentration of GR on proangiogenesis effect was determined to be 100 μg/mL. Ten compounds, including gastrodin, parishin E, stigmasterol, p-hydroxybenzyl alcohol, citric acid, etc., were identified to have high correlation coefficients with proangiogenic activity. Furthermore, the network pharmacologic analysis of these compounds revealed that the compounds systematically regulate the formation of new blood vessels via networked vital targets and signalling pathways.
GR can promote the growth of blood vessels, ten chemical components discovered contribute to this proangiogenesis activity. These chemical markers of GR thus provide a foundation for further studies on medicinal substances and quality evaluation of GR, also providing a scientific basis for modern interpretation of the processing theory of traditional Chinese medicine.
血管生成与多种疾病密切相关，基于血管生成的治疗方法受到了广泛关注。研究表明，天麻（Gastrodia elata）的使用可以通过刺激血管生成来治疗缺血性心血管疾病和动脉粥样硬化。
本研究测试了从天麻中分离出的一组化学标志物对血管生成的影响。
通过设置空白对照组、模型组、阳性对照组和治疗组（0.1、1 和 100 μg/mL RGP），利用斑马鱼模型来评估血管生成。采用灰色关联分析（GCA）计算各化合物在液相色谱-飞行时间质谱（LC-TOF-MS）中的峰面积与生物活性之间的相关系数，列出关联度>0.9 的前 10 个化合物。
确定天麻对促血管生成作用的最佳终浓度为 100 μg/mL。鉴定出 10 种化合物，包括天麻素、对羟基苯甲醇、柠檬酸等，与促血管生成活性具有较高的相关系数。此外，这些化合物的网络药理学分析表明，这些化合物通过网络关键靶点和信号通路系统地调节新血管的形成。
天麻能促进血管生长，发现的 10 种化学成分具有促血管生成活性。这些天麻的化学标志物为进一步研究药用物质和天麻质量评价提供了基础，也为现代阐释中药炮制理论提供了科学依据。
