Fehér J, Láng I, Nékám K, Müzes G, Deák G
Second Department of Medicine, Semmelweis University Medical School, Budapest, Hungary.
Acta Med Hung. 1988;45(3-4):265-76.
The in vitro and in vivo effects of three hepatoprotective antioxidants (silymarin, (+)cyanidanol-3 and 4-amino-5-imidazole-carboxamide-phosphate) on the expression and activity of superoxide dismutase (SOD) enzyme were studied in erythrocytes and lymphocytes from patients with alcoholic cirrhosis. In vitro incubation with the drugs in a concentration corresponding to the usual therapeutic dosage markedly increased (i) the SOD expression of lymphocytes as measured by flow-cytofluorimetry following staining with monoclonal anti-Cu, Zn-SOD-antibody and FITC-conjugated anti-mouse Ig, as well as (ii) erythrocyte and lymphocyte SOD activities. In vivo treatment also restored the originally low SOD activity and expression of the patients' lymphocytes and erythrocytes. The data indirectly suggest that antioxidant activity might be one of the important factors in the hepatoprotective action of these agents.
研究了三种保肝抗氧化剂（水飞蓟素、（+）矢车菊素-3和4-氨基-5-咪唑甲酰胺磷酸盐）对酒精性肝硬化患者红细胞和淋巴细胞中超氧化物歧化酶（SOD）酶表达及活性的体外和体内作用。用对应于常用治疗剂量的浓度的药物进行体外孵育，显著增加了：（i）用单克隆抗铜、锌超氧化物歧化酶抗体染色并用异硫氰酸荧光素（FITC）偶联的抗小鼠免疫球蛋白进行检测，通过流式细胞荧光测定法测得的淋巴细胞超氧化物歧化酶表达，以及（ii）红细胞和淋巴细胞的超氧化物歧化酶活性。体内治疗也恢复了患者淋巴细胞和红细胞原本较低的超氧化物歧化酶活性及表达。数据间接表明抗氧化活性可能是这些药物保肝作用的重要因素之一。
