Fehér J, Láng I, Deák G, Cornides A, Nékám K, Gergely P
II. Department of Medicine, Semmelweis University, Budapest, Hungary.
Tokai J Exp Clin Med. 1986;11 Suppl:121-34.
In vitro and in vivo effects of four hepatoprotective agents: silymarin (LegalonR), (+)-cyanidanol-3 (CatergenR), 6,6-methylene-bis(2,2,4-trimethyl-1, 2-dihydroquinoline) (MTDQ), and 4,5-amino-imidazole-carboxamide-phosphate (Aica-P) on the expression and activity of superoxide dismutase enzyme and on certain cellular immune reactions were studied in lymphocytes (and erythrocytes) from cirrhotic patients and from healthy control subjects. In vitro incubation with these drugs inhibited lectin-induced lymphocyte transformation and some of them decreased the antibody-dependent, spontaneous, and lectin-induced lymphocytotoxicity. MTDQ, silymarin and Aica-P enhanced the superoxide dismutase activity of erythrocytes and lymphocytes and the two latter and (+)-cyanidanol-3 increased the superoxide expression of lymphocytes as measured by flow cytofluorometry. In vivo treatment with Aica-P restored the originally low lymphocyte transformation values of patients' lymphocytes. Our results indirectly suggest that both antioxidant and immunomodulatory activities might be important factors in the hepatoprotective action of these drugs.
水飞蓟素（利加隆）、（+）-儿茶素-3（卡特金）、6,6-亚甲基双（2,2,4-三甲基-1,2-二氢喹啉）（MTDQ）和4,5-氨基咪唑甲酰胺磷酸盐（Aica-P）对肝硬化患者和健康对照者淋巴细胞（及红细胞）中超氧化物歧化酶的表达和活性以及某些细胞免疫反应的体外和体内作用。用这些药物进行体外孵育可抑制凝集素诱导的淋巴细胞转化，其中一些药物还可降低抗体依赖性、自发性和凝集素诱导的淋巴细胞毒性。MTDQ、水飞蓟素和Aica-P可增强红细胞和淋巴细胞的超氧化物歧化酶活性，后两者及（+）-儿茶素-3可通过流式细胞荧光测定法增加淋巴细胞的超氧化物表达。用Aica-P进行体内治疗可恢复患者淋巴细胞原本较低的转化值。我们的结果间接表明，抗氧化和免疫调节活性可能是这些药物保肝作用的重要因素。
