Nicolescu Alexandru, Babotă Mihai, Ilea Maria, Dias Maria Inês, Calhelha Ricardo C, Gavrilaș Laura, Rocchetti Gabriele, Crișan Gianina, Mocan Andrei, Barros Lillian, Pârvu Alina Elena
Department of Pharmaceutical Botany, "Iuliu Hațieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Department of Pathophysiology, Faculty of Medicine, "Iuliu Hațieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Front Pharmacol. 2022 Aug 19;13:975800. doi: 10.3389/fphar.2022.975800. eCollection 2022.
, also known as the glutinous sage, has been used in Romanian folk medicine in the treatment of inflammation, injuries, and mild infections. However, there is no direct scientific evidence to demonstrate these activities. The present research was based on evaluating antioxidant, antiproliferative, and α-glucosidase inhibitory activity of extracts, as well as the anti-inflammatory activity. Infusions and 70% () ethanol solution extracts of stems and leaves, collected from two different locations in Romania, were prepared. Ten phenolic compounds were identified and quantified using the LC-DAD-ESI/MS method, and total phenolic and flavonoid content, as well as antioxidant (DPPH, ABTS, and FRAP assays), antiproliferative, anti-inflammatory and alpha-glucosidase inhibitory activities were determined. A rat model of induced inflammation with turpentine oil was used for the examination of effects of the extracts, using diclofenac as an anti-inflammatory control. The highest inhibitory α-glucosidase activity was determined to be IC = 0.546 mg/ml for the hydroalcoholic extract made with plant material collected on the road to Sighișoara. The highest cytotoxic activity against HepG2 cell line was determined to be GI = 131.68 ± 5.03 μg/ml, for the hydroalcoholic extract made with plant material from Sighișoara. administration of extract (200 mg lyophilized powder/ml) showed a significant reduction of NO production. Our findings indicate that extracts exhibit antioxidant, α-glucosidase inhibitory activity, as well as a modest cytotoxic effect on HepG2 cell line. By administration, the extracts show anti-inflammatory and antioxidant activity, which correlates with the traditional use of the species. The environmental conditions seemed to induce important changes in the chemical composition and the bioactivity of the herbal preparations derived from
[植物名称]，也被称为糯米鼠尾草，已被用于罗马尼亚民间医学中治疗炎症、损伤和轻度感染。然而，尚无直接科学证据证明这些功效。本研究基于评估[植物名称]提取物的抗氧化、抗增殖和α - 葡萄糖苷酶抑制活性，以及抗炎活性。制备了从罗马尼亚两个不同地点采集的[植物名称]茎和叶的浸剂和70%（体积分数）乙醇溶液提取物。使用LC - DAD - ESI/MS方法鉴定并定量了10种酚类化合物，并测定了总酚和黄酮含量，以及抗氧化（DPPH、ABTS和FRAP测定）、抗增殖、抗炎和α - 葡萄糖苷酶抑制活性。使用松节油诱导炎症的大鼠模型来检测提取物的效果，以双氯芬酸作为抗炎对照。对于在通往锡吉什瓦拉的道路上采集的植物材料制成的水醇提取物，测定其最高α - 葡萄糖苷酶抑制活性为IC50 = 0.546 mg/ml。对于来自锡吉什瓦拉的植物材料制成的水醇提取物，测定其对HepG2细胞系的最高细胞毒性活性为GI50 = 131.68 ± 5.03 μg/ml。给予提取物（200 mg冻干粉末/ml）显示一氧化氮产生显著减少。我们的研究结果表明，[植物名称]提取物具有抗氧化、α - 葡萄糖苷酶抑制活性，以及对HepG2细胞系有适度的细胞毒性作用。通过给予提取物，其显示出抗炎和抗氧化活性，这与该物种的传统用途相关。环境条件似乎在源自[植物名称]的草药制剂的化学成分和生物活性方面引起了重要变化。
