State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210023, China.
Henan Provincial Key Laboratory of Children's Genetics and Metabolic Diseases, Children's Hospital Affiliated to Zhengzhou University, Zhengzhou, 450018, China.
Talanta. 2020 Sep 1;217:121087. doi: 10.1016/j.talanta.2020.121087. Epub 2020 Apr 28.
Sulfur-containing species are essential in the composition and the metabolism of the organisms, thus developing a full set of implements to cover all of them is still a favorable choice. Herein, we chose imidazo [1,5-α]pyridine moiety as the basic fluorophore for the detection of sulfite, and preliminarily completed the toolset since biothiols (GSH, Cys, Hcy), HS, and PhSH could be detected by sensors based on the same backbone. The designed sensor, IPD-SFT, with structural novelty and large Stokes shift (130 nm), indicated the most attractive advantages of remarkably rapid response period (within 1 min) and high selectivity for sulfite from all the sulfur-containing species. Other practical properties included high sensitivity (LOD = 50 nM) and wide pH adaptability (5.0-11.0). Furthermore, IPD-SFT could monitor both exogenous and endogenous sulfite. It not only raised a potential tool for sulfite detection, but also preliminarily completed the toolset for all the sulfur-containing species. The development of such toolsets might reveal the sulfur-containing metabolism and corresponding physiology and pathological procedures.
含硫物种是生物组成和代谢所必需的，因此开发一整套工具来涵盖所有这些物种仍然是一个有利的选择。在这里，我们选择咪唑并[1,5-α]吡啶部分作为检测亚硫酸盐的基本荧光团，并初步完成了工具集，因为基于相同骨架的传感器可以检测生物硫醇（GSH、Cys、Hcy）、HS 和 PhSH。设计的传感器 IPD-SFT 具有结构新颖和大斯托克斯位移（130nm）的特点，表明其具有最吸引人的优势，包括对亚硫酸盐的快速响应时间（在 1 分钟内）和高选择性，以及对所有含硫物种的高选择性。其他实际性能包括高灵敏度（LOD=50nM）和宽 pH 适应性（5.0-11.0）。此外，IPD-SFT 可以监测外源性和内源性亚硫酸盐。它不仅为亚硫酸盐检测提供了一种潜在的工具，而且还初步完成了所有含硫物种的工具集。此类工具集的开发可能揭示含硫代谢及其相应的生理和病理过程。
