Kong Xiaojian, Zhao Jie, Yang Lei, Wang Feng, Sun Zhiwei
School of Chemistry, Chemical Engineering and Materials, Jining University, Qufu, 273155, China.
Key Laboratory of Life-Organic Analysis of Shandong Province, Qufu Normal University, Qufu, China.
Anal Bioanal Chem. 2024 Jan;416(2):329-339. doi: 10.1007/s00216-023-05039-1. Epub 2023 Nov 21.
Phosgene is a highly concealed and highly toxic gas that seriously threatens human health and public security. Therefore, the detection of phosgene is of great significance to world security. Herein, a new type of fluorescent probe based on 2-(2-aminophenyl) imidazo [1,5-a] pyridine is reported for the rapid detection of phosgene. The probe itself only emits a faint green fluorescence, while phosgene allows it to produce a strong blue fluorescence. During the recognition process, phosgene interacts simultaneously with both amino site and imidazole moiety in the probe molecule, resulting in a four-ring-fused rigid structure with high fluorescence quantum yield. The probe not only has the characteristics of high efficiency, high sensitivity (detection limit 2.68 nM), and high selectivity, but also has remarkable spectral changes. Finally, a portable test strip is used to detect phosgene in the gas phase, and the fluorescent color change of the test strip can be easily observed. The most exciting thing is that the portable test strip with the probe PMPY-NH can produce a strong fluorescence response to 1 ppm of phosgene, which is far lower than the level of phosgene that seriously threatens to human health.
光气是一种极具隐蔽性和高毒性的气体，严重威胁人类健康和公共安全。因此，光气的检测对世界安全具有重要意义。在此，报道了一种基于2-(2-氨基苯基)咪唑并[1,5-a]吡啶的新型荧光探针用于光气的快速检测。该探针本身仅发出微弱的绿色荧光，而光气能使其产生强烈的蓝色荧光。在识别过程中，光气与探针分子中的氨基位点和咪唑部分同时相互作用，形成具有高荧光量子产率的四环稠合刚性结构。该探针不仅具有高效、高灵敏度（检测限2.68 nM）和高选择性的特点，而且具有显著的光谱变化。最后，使用便携式测试条检测气相中的光气，可轻松观察到测试条的荧光颜色变化。最令人兴奋的是，带有探针PMPY-NH的便携式测试条对1 ppm的光气能产生强烈的荧光响应，这一浓度远低于严重威胁人类健康的光气水平。
